Invitrogen

XPF Monoclonal Antibody (51)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

7 References

View all (30) XPF antibodies

Datasheet

Datasheet

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XPF Antibody — Published figure using XPF monoclonal antibody (Product # MA5-12060)

XPF Antibody in Western Blot (WB) — Western blot analysis was performed on nuclear extracts (30 µg lysate) of A-375 (Lane 1), A-431 (Lane 2), SK-OV-3 (Lane 3), Sf9 (Lane 4), Hep G2 (Lane 5), HeLa (Lane 6), A549 (Lane 7) and Jurkat (Lane 8). The blot was probed with Anti-XPF Mouse Monoclonal Antibody (Product # MA5-12060, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 104 kDa band corresponding to XPF was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

XPF Antibody in ChIP assay (ChIP) — Enrichment of endogenous XPF protein at specific gene loci using Anti-XPF Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-XPF Mouse Monoclonal Antibody (Product # MA5-12060, 3 µg) on sheared chromatin from 2 million HeLa cells treated with UV (2 hours) using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over EE1F1A, GAPDH promoter (active) and GAPDH gene, SAT alpha (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

XPF Antibody in Relative expression — Antibody specificity was demonstrated by detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using Anti-XPF Monoclonal Antibody (Product # MA5-12060) using PCR primer pairs over EE1F1A, GAPDH promoter (active) and GAPDH gene, SAT alpha (inactive). {RE}

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

1.5 µg/10^6 cells

Western Blot (WB)

1-3 µg/mL

Published Applications

Western Blot (WB)

See 5 publications below

Immunocytochemistry (ICC)

See 1 publication below

Neutralization (Neu)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Recombinant human XPF protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10987557

Product Specific Information

MA5-12060 targets XPF in WB applications and shows reactivity with Human samples.

The MA5-12060 immunogen is recombinant human XPF protein.

Target Information

The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: complementation group F; complementing defective; DNA excision repair protein ERCC-4; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair; excision-repair, complementing defective, in Chinese hamster; in Chinese hamster; xeroderma pigmentosum; Xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F

Gene Aliases: ERCC11; ERCC4; FANCQ; RAD1; XFEPS; XPF

UniProt ID: (Human) Q92889

Entrez Gene ID: (Human) 2072

Molecular Function: DNA binding protein endodeoxyribonuclease nuclease nucleic acid binding