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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human XPG (Xeroderma Pigmentosum type G) protein, expressed in baculovirus.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/mg of protein lysate|
|Western Blot (WB)||1:625-1:1250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26186 detects XPG from human samples.
MA1-26186 has been successfully used in IP and Western blot procedures.
The MA1-26186 immunogen is recombinant human XPG (Xeroderma Pigmentosum type G) protein, expressed in baculovirus.
Excision repair cross-complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G) is involved in excision repair of UV-induced DNA damage. Mutations cause Cockayne syndrome, which is characterized by severe growth defects, mental retardation, and cachexia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been described, but the biological validity of all variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.
MA1-26186 was used in immunocytochemistry to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair
|Zhu Q,Wani G,Sharma N,Wani A||DNA repair (11:942)||2012|
Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state.
MA1-26186 was used in immunocytochemistry to study the functional association between XP-G/CS mutations and the disassembly state of TFIIH
|Arab HH,Wani G,Ray A,Shah ZI,Zhu Q,Wani AA||PloS one (5:null)||2010|
Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH.
MA1-26186 was used in immunocytochemistry to study the role of TFIIH in the function of nucleotide excision repair endonuclease XPG
|Zotter A,Luijsterburg MS,Warmerdam DO,Ibrahim S,Nigg A,van Cappellen WA,Hoeijmakers JH,van Driel R,Vermeulen W,Houtsmuller AB||Molecular and cellular biology (26:8868)||2006|
Spatiotemporal dynamics of p21CDKN1A protein recruitment to DNA-damage sites and interaction with proliferating cell nuclear antigen.
MA1-26186 was used in immunocytochemistry to study the dynamics of cyclin-dependent kinase inhibitor p21CDKN1A recruitment to DNA-damage sites
|Perucca P,Cazzalini O,Mortusewicz O,Necchi D,Savio M,Nardo T,Stivala LA,Leonhardt H,Cardoso MC,Prosperi E||Journal of cell science (119:1517)||2006|
p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG.
MA1-26186 was used in western blot to study the acetylation of XPG by p300/CBP acetyl transferases and the potential modulatory role of PCNA-p21
|Tillhon M,Cazzalini O,Nardo T,Necchi D,Sommatis S,Stivala LA,Scovassi AI,Prosperi E||DNA repair (11:844)||2012|
Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair.
MA1-26186 was used in western blot to study the role of the cell cycle inhibitor p21(CDKN1A) in regulating nucleotide excision repair
|Cazzalini O,Perucca P,Savio M,Necchi D,Bianchi L,Stivala LA,Ducommun B,Scovassi AI,Prosperi E||Nucleic acids research (36:1713)||2008|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA1-26186 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
COFS3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; ERCM2; excision repair cross-complementation group 5; excision repair cross-complementing rodent repair deficiency, complementation group 5; RP11-484I6.5; UVDR; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum, complementation group G; XPG; XPG-complementing protein; XPGC
COFS3; ERCC5; ERCC5-201; ERCM2; UVDR; XPG; XPGC