Invitrogen

alpha Internexin Monoclonal Antibody (2E3)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

11 References

View all (22) alpha Internexin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

alpha Internexin Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 5

alpha Internexin Antibody (32-3600) in IHC

Mouse anti-Alpha-Internexin (2E3) stained paraganglioma.

alpha Internexin Antibody in Western Blot (WB)

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Product Details

32-3600

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

1-5 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E3

Immunogen

Full-length recombinant rat alpha-internexin protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533075

Product Specific Information

This antibody is purified from mouse ascites fluid and is specific for the 66 kDa alpha-internexin protein. 2E3 antibody has stained human neuron and nerve fiber in human cerebral cortex. It has also stained glioma, ganglioneuroma, pheochromocytoma, and paraganglioma in human tissue. It is recommended that FFPE tissues are pretreated with HIER using citrate buffer, pH 6.

Target Information

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis.

Human mutation

van der Zee J,Gijselinck I,Van Mossevelde S,Perrone F,Dillen L,Heeman B,Bäumer V,Engelborghs S,De Bleecker J,Baets J,Gelpi E,Rojas-García R,Clarimón J,Lleó A,Diehl-Schmid J,Alexopoulos P,Perneczky R,Synofzik M,Just J,Schöls L,Graff C,Thonberg H,Borroni B,Padovani A,Jordanova A,Sarafov S,Tournev I,de Mendonça A,Miltenberger-Miltényi G,Simões do Couto F,Ramirez A,Jessen F,Heneka MT,Gómez-Tortosa E,Danek A,Cras P,Vandenberghe R,De Jonghe P,De Deyn PP,Sleegers K,Cruts M,Van Broeckhoven C,Goeman J,Nuytten D,Smets K,Robberecht W,Damme PV,Bleecker J,Santens P,Dermaut B,Versijpt J,Michotte A,Ivanoiu A,Deryck O,Bergmans B,Delbeck J,Bruyland M,Willems C,Salmon E,Pastor P,Ortega-Cubero S,Benussi L,Ghidoni R,Binetti G,Hernández I,Boada M,Ruiz A,Sorbi S,Nacmias B,Bagnoli S,Sorbi S,Sanchez-Valle R,Llado A,Santana I,Rosário Almeida M,Frisoni GB,Maetzler W,Matej R,Fraidakis MJ,Kovacs GG,Fabrizi GM,Testi S

323600 was used in immunohistochemistry - paraffin section to examine the mutation spectrum of the TANK-Binding Kinase 1 gene and its associated phenotypic spectrum in patients with FTD, ALS or FTD plus ALS

Wed Mar 01 00:00:00 EST 2017

Neuropathological criteria of anti-IgLON5-related tauopathy.

Acta neuropathologica

Gelpi E,Höftberger R,Graus F,Ling H,Holton JL,Dawson T,Popovic M,Pretnar-Oblak J,Högl B,Schmutzhard E,Poewe W,Ricken G,Santamaria J,Dalmau J,Budka H,Revesz T,Kovacs GG

323600 was used in immunohistochemistry - paraffin section to detail the neuropathological features of the anti-IgLON5 syndrome in order to diagnose patients

Sat Oct 01 00:00:00 EDT 2016

An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions.

Neuropathology : official journal of the Japanese Society of Neuropathology

Inoue K,Fujimura H,Ueda K,Matsumura T,Itoh K,Sakoda S

32-3600 was used in immunohistochemistry - paraffin section to report an autopsy case of neuronal intermediate filament inclusion disease.

Tue Dec 01 00:00:00 EST 2015

PRKAR1B mutation associated with a new neurodegenerative disorder with unique pathology.

Brain : a journal of neurology

Wong TH,Chiu WZ,Breedveld GJ,Li KW,Verkerk AJ,Hondius D,Hukema RK,Seelaar H,Frick P,Severijnen LA,Lammers GJ,Lebbink JH,van Duinen SG,Kamphorst W,Rozemuller AJ,Bakker EB,Neumann M,Willemsen R,Bonifati V,Smit AB,van Swieten J

32-3600 was used in western blot to study the association of PRKAR1B mutation with a new neurodegenerative disorder

Thu May 01 00:00:00 EDT 2014

Frontotemporal dementia-amyotrophic lateral sclerosis syndrome locus on chromosome 16p12.1-q12.2: genetic, clinical and neuropathological analysis.

Acta neuropathologica

Dobson-Stone C,Luty AA,Thompson EM,Blumbergs P,Brooks WS,Short CL,Field CD,Panegyres PK,Hecker J,Solski JA,Blair IP,Fullerton JM,Halliday GM,Schofield PR,Kwok JB

32-3600 was used in Immunohistochemistry to suggest that a locus on chromosome 16p12.1-16q12.2 is responsible for an unusual cluster of neurodegenerative phenotypes.

Mon Apr 01 00:00:00 EDT 2013

Gliosarcoma with ependymal and PNET-like differentiation.

Clinical neuropathology

Shintaku M,Yoneda H,Hirato J,Nagaishi M,Okabe H

32-3600 was used in immunohistochemistry to characterize a rare case of gliosarcoma

Thu Mar 13 00:00:00 EDT 2014

An MND/ALS phenotype associated with C9orf72 repeat expansion: abundant p62-positive, TDP-43-negative inclusions in cerebral cortex, hippocampus and cerebellum but without associated cognitive decline.

Neuropathology : official journal of the Japanese Society of Neuropathology

Troakes C,Maekawa S,Wijesekera L,Rogelj B,Siklós L,Bell C,Smith B,Newhouse S,Vance C,Johnson L,Hortobágyi T,Shatunov A,Al-Chalabi A,Leigh N,Shaw CE,King A,Al-Sarraj S

32-3600 was used in immunohistochemistry - paraffin section to study 4 cases of chromosome 9-linked motor neuron disease/amyotrophic lateral sclerosis.

Mon Oct 01 00:00:00 EDT 2012

FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations.

Brain : a journal of neurology

Neumann M,Bentmann E,Dormann D,Jawaid A,DeJesus-Hernandez M,Ansorge O,Roeber S,Kretzschmar HA,Munoz DG,Kusaka H,Yokota O,Ang LC,Bilbao J,Rademakers R,Haass C,Mackenzie IR

Thu Sep 01 00:00:00 EDT 2011

FET proteins TAF15 and EWS are selective markers that distinguish FTLD with FUS pathology from amyotrophic lateral sclerosis with FUS mutations.

Brain : a journal of neurology

Neumann M,Bentmann E,Dormann D,Jawaid A,DeJesus-Hernandez M,Ansorge O,Roeber S,Kretzschmar HA,Munoz DG,Kusaka H,Yokota O,Ang LC,Bilbao J,Rademakers R,Haass C,Mackenzie IR

Thu Sep 01 00:00:00 EDT 2011

What's in a name? Neuronal intermediate filament inclusion disease (NIFID), frontotemporal lobar degeneration-intermediate filament (FTLD-IF) or frontotemporal lobar degeneration-fused in sarcoma (FTLD-FUS)?

Journal of neurology, neurosurgery, and psychiatry

Menon R,Baborie A,Jaros E,Mann DM,Ray PS,Larner AJ

Thu Dec 01 00:00:00 EST 2011

Cytoskeletal organization of the developing mouse olfactory nerve layer.

The Journal of comparative neurology

Akins MR,Greer CA

32-3600 was used in immunohistochemistry to test if the cytoskeletal elements of olfactory sensory neuron axons may be are differentially expressed across the olfactory nerve layer.

Tue Jan 10 00:00:00 EST 2006

Cytoskeletal organization of the developing mouse olfactory nerve layer.

The Journal of comparative neurology

Akins MR,Greer CA

32-3600 was used in immunohistochemistry to test if the cytoskeletal elements of olfactory sensory neuron axons may be are differentially expressed across the olfactory nerve layer.

Tue Jan 10 00:00:00 EST 2006

Bioinformatics

Protein Aliases: 66 kDa neurofilament protein; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; internexin neuronal intermediate filament protein, alpha; internexin, alpha; MGC12702; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; NF-66

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Gene Aliases: AV028420; INA; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1

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UniProt ID: (Human) Q16352, (Mouse) P46660

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Entrez Gene ID: (Human) 9118, (Rabbit) 100348512, (Mouse) 226180, (Rat) 24503

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Function(s)

structural constituent of cytoskeleton structural molecule activity protein binding protein heterodimerization activity

Process(es)

multicellular organism development nervous system development cell differentiation intermediate filament cytoskeleton organization neurofilament cytoskeleton organization substantia nigra development tissue regeneration

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Internexin Monoclonal Antibody (2E3)

alpha Internexin Antibody (32-3600) in IHC

Product Details

32-3600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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