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|Tested species reactivity||Human|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full-length recombinant rat alpha-internexin protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1.0 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||0.1-0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Alpha internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with some species variability, although the real molecular weight is about 55kDa; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cells committed to the neuronal lineage. In addition, recent studies show a marked up-regulation of a-internexin during neuronal regeneration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PRKAR1B mutation associated with a new neurodegenerative disorder with unique pathology.
32-3600 was used in western blot to study the association of PRKAR1B mutation with a new neurodegenerative disorder
|Wong TH,Chiu WZ,Breedveld GJ,Li KW,Verkerk AJ,Hondius D,Hukema RK,Seelaar H,Frick P,Severijnen LA,Lammers GJ,Lebbink JH,van Duinen SG,Kamphorst W,Rozemuller AJ,Bakker EB,Neumann M,Willemsen R,Bonifati V,Smit AB,van Swieten J||Brain : a journal of neurology (137:1361)||2014|
Gliosarcoma with ependymal and PNET-like differentiation.
32-3600 was used in immunohistochemistry to characterize a rare case of gliosarcoma
|Shintaku M,Yoneda H,Hirato J,Nagaishi M,Okabe H||Clinical neuropathology (32:508)||2013|
Cytoskeletal organization of the developing mouse olfactory nerve layer.
32-3600 was used in immunohistochemistry to test if the cytoskeletal elements of olfactory sensory neuron axons may be are differentially expressed across the olfactory nerve layer.
|Akins MR,Greer CA||The Journal of comparative neurology (494:358)||2006|
An MND/ALS phenotype associated with C9orf72 repeat expansion: abundant p62-positive, TDP-43-negative inclusions in cerebral cortex, hippocampus and cerebellum but without associated cognitive decline.
32-3600 was used in immunohistochemistry - paraffin section to study 4 cases of chromosome 9-linked motor neuron disease/amyotrophic lateral sclerosis.
|Troakes C,Maekawa S,Wijesekera L,Rogelj B,Siklós L,Bell C,Smith B,Newhouse S,Vance C,Johnson L,Hortobágyi T,Shatunov A,Al-Chalabi A,Leigh N,Shaw CE,King A,Al-Sarraj S||Neuropathology : official journal of the Japanese Society of Neuropathology (32:505)||2012|
What's in a name? Neuronal intermediate filament inclusion disease (NIFID), frontotemporal lobar degeneration-intermediate filament (FTLD-IF) or frontotemporal lobar degeneration-fused in sarcoma (FTLD-FUS)?
||Menon R,Baborie A,Jaros E,Mann DM,Ray PS,Larner AJ||Journal of neurology, neurosurgery, and psychiatry (82:1412)||2011|
NF-66, NEF5, TXBP-1
66 kDa neurofilament protein, alpha-Inx, alpha-internexin, neurofilament 5 (66kD), neurofilament-66, tax-binding protein, Alpha-internexin, Alpha-Inx, Neurofilament-66, NF-66, NEF5