Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
1:500-1:3000 | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to the C-terminal residues S(129) E E G Y Q D Y E P E A(140) of human alpha synuclein protein. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
Conc. Not Determined |
Storage buffer |
whole serum |
Contains |
no preservative |
Storage conditions |
-20°C |
RRID |
AB_2192944 |
PA1-18264 detects alpha Synuclein from human, mouse, and rat samples.
PA1-18264 has been successfully used in immunohistochemistry and Western blot procedures.
The PA1-18264 immunogen is a synthetic peptide corresponding to the C-terminal residues S(129) E E G Y Q D Y E P E A(140) of human alpha synuclein protein, conjugated to Blue Carrier Protein. The sequence is conserved in monkey and pig.
After reconstitution, store aliquots at -20 C or 4 C with appropriate antibacterial agent. Avoid freeze/thaw cycles.
Reconstitute with 100 µL of distilled water. Glycerol (1:1) may be added for additional stability.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Alpha-Synuclein belongs to the Synuclein family, which includes beta and gamma Synuclein, and is predominantly expressed in neurons, and concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. Alpha-Synuclein is a small, presynaptic protein that is the major nonamyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. Alpha-Synuclein is involved in the formation of SNARE complexes, and most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson's disease (PD) and other neurodegenerative disorders. Mutation of the alpha-synuclein gene is associated with familial forms of PD. Alpha-Synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk and Tat-binding protein 1. The alpha and beta synucleins are found primarily in brain tissue where they are seen mainly in neuronal cytosol and presynaptic terminals. The antibody specific for alpha-synuclein does not cross react with beta-synuclein and will be useful for the confirmation of Parkinson's disease and Lewy body dementia and other studies for the localization and detection of alpha-synuclein. Four alternatively spliced transcripts that encode two different isoforms of the alpha-synuclein protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)
Gene Aliases: alphaSYN; NACP; PARK1; PARK4; PD1; SNCA; Syn
UniProt ID: (Human) P37840, (Rat) P37377, (Mouse) O55042
Entrez Gene ID: (Human) 6622, (Rat) 29219, (Mouse) 20617
Molecular Function:
membrane trafficking regulatory protein
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support