Immunofluorescent staining of mouse brain (locus coeruleus) double-labeled with Ms anti-Nitrated a-Synuclein (Cat. no. 35-8300, red) and anti-Tyrosine Hydroxylase (green). Image courtesy of John Trojanowski, MD, PhD, University of Pennsylvania School of Medicine, PA.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full-length oxidized human alpha-Synuclein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Alpha-Synuclein belongs to the Synuclein family, which also includes beta and gamma Synuclein, and is predominantly expressed in neurons, concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. Alpha-Synuclein is a small, presynaptic protein that is the major nonamyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. It is involved in the formation of SNARE complexes. Most significantly, aggregated Alpha-Synuclein is one of the major components found in the Lewy bodies that occur in Parkinson's disease (PD) and other neurodegenerative disorders. Mutation of the alpha-synuclein gene is associated with familial forms of PD. Alpha-Synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk and Tat-binding protein 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Age-dependent ¿-synuclein aggregation in the Microcebus murinus lemur primate.
35-8300 was used in immunohistochemistry to assess Microcebusmurinus as a model of age-related synucleinopathy
|Canron MH,Perret M,Vital A,Bézard E,Dehay B||Scientific reports (2:null)||2012|
||Novel antibodies to synuclein show abundant striatal pathology in Lewy body diseases.||Duda JE,Giasson BI,Mabon ME,Lee VM,Trojanowski JQ||Annals of neurology (52:205)||2002|
|Human||Not Cited||Novel antibodies to synuclein show abundant striatal pathology in Lewy body diseases.||Duda JE,Giasson BI,Mabon ME,Lee VM,Trojanowski JQ||Annals of neurology (52:205)||2002|