Invitrogen

alpha Tubulin Polyclonal Antibody

Product Image Gallery

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-19489) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

alpha Tubulin Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), NIH/3T3 (Lane 4), HT-29 (Lane 5), Neuro-2a (Lane 6) and tissue extract of Rat Brain (Lane 7). The blot was probed with Rabbit Anti-alpha Tubulin Polyclonal Antibody (Product # PA5-19489, 0.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to alpha Tubulin was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

PA5-19489

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5 µg/mL	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1-5 µg/mL	View 5 publications 5 publications
Flow Cytometry (Flow)	0.01 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Bovine, Chicken, Deer, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.02% sodium azide
Storage conditions	-20°C or -80°C if preferred
RRID	AB_10984311

Product Specific Information

PA5-19489 targets alpha Tubulin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with chicken, Indian muntjac, Chinese hamster, human, fruit fly, rat, African Green Monkey, and mouse samples. Perform heat mediated antigen retrieval via the pressure cooker method before beginning immunohistochemistry staining protocol.

The PA5-19489 immunogen is synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.

PA5-19489 detects alpha Tubulin which has a predicted molecular weight of approximately 50 kDa.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A cytoskeletal function for PBRM1 reading methylated microtubules.

Science advances

Karki M,Jangid RK,Anish R,Seervai RNH,Bertocchio JP,Hotta T,Msaouel P,Jung SY,Grimm SL,Coarfa C,Weissman BE,Ohi R,Verhey KJ,Hodges HC,Burggren W,Dere R,Park IY,Prasad BVV,Rathmell WK,Walker CL,Tripathi DN

PA5-19489 was used in Immunocytochemistry to reveal PBRM1 functions beyond chromatin remodeling with domains that allow it to integrate chromatin and cytoskeletal activity via its acetyl-binding BD and methyl-binding BAH domains, respectively.

Thu Apr 01 00:00:00 EDT 2021

The MiDAC histone deacetylase complex is essential for embryonic development and has a unique multivalent structure.

Nature communications

Turnbull RE,Fairall L,Saleh A,Kelsall E,Morris KL,Ragan TJ,Savva CG,Chandru A,Millard CJ,Makarova OV,Smith CJ,Roseman AM,Fry AM,Cowley SM,Schwabe JWR

PA5-19489 was used in Immunocytochemistry-immunoflourescence to investigate the role of MiDAC during mitosis in cancer cell lines using mouse models.

Fri Jun 26 00:00:00 EDT 2020

Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors.

Cell reports

Roscioli E,Germanova TE,Smith CA,Embacher PA,Erent M,Thompson AI,Burroughs NJ,McAinsh AD

PA5-19489 was used in Immunocytochemistry-immunoflourescence to define how proteins from six major complexes and the checkpoint proteins Bub1, Mad1, and Mad2 are organized in the human kinetochore.

Tue Apr 28 00:00:00 EDT 2020

Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic α-helix.

The EMBO journal

Burgess SG,Mukherjee M,Sabir S,Joseph N,Gutiérrez-Caballero C,Richards MW,Huguenin-Dezot N,Chin JW,Kennedy EJ,Pfuhl M,Royle SJ,Gergely F,Bayliss R

Published figure using alpha Tubulin polyclonal antibody (Product # PA5-19489) in Immunofluorescence

Fri Apr 13 00:00:00 EDT 2018

Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs).

Aging

Colasuonno F,Borghi R,Niceforo A,Muzzi M,Bertini E,Di Giulio A,Moreno S,Compagnucci C

Published figure using alpha Tubulin polyclonal antibody (Product # PA5-19489) in Western Blot

Mon Oct 23 00:00:00 EDT 2017

FGFR3-TACC3 cancer gene fusions cause mitotic defects by removal of endogenous TACC3 from the mitotic spindle.

Open biology

Sarkar S,Ryan EL,Royle SJ

Published figure using alpha Tubulin polyclonal antibody (Product # PA5-19489) in Western Blot

Tue Aug 01 00:00:00 EDT 2017

The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle.

eLife

Nixon FM,Gutiérrez-Caballero C,Hood FE,Booth DG,Prior IA,Royle SJ

PA5-19489 was used in immunocytochemistry to describe how kinetochore fibers and microtubules are associated

Fri Jun 19 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: &agr-tubulin 84B; alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alpha-Tubulin 84B; Alpha-tubulin I; Alpha-tubulin II; Alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; AT25469p; CG1913-PA; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha; tubulin alpha 1c; tubulin alpha 6; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; Tubulin alpha-6 chain; tubulin alpha-8 chain; Tubulin alpha-ubiquitous chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin B-alpha-1; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; Tubulin-alpha1; tubulin1alpha; tubulin84B; unnamed protein product

Gene Aliases: 1t; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; Alpha-Tubulin84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; B-ALPHA-1; bA408E5.3; bcm948; BEST:LD32507; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; H2-ALPHA; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; LIS3; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; T; Talpha1; TBA1_DROME; Tub; Tub 84B; Tub1C; Tub84B; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; Tuba84B; Tubalpha1; tubulin

UniProt ID: (Human) Q71U36, (Bovine) P81947, (Human) P68363, (Bovine) Q3ZCJ7, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Bovine) Q32KN8, (Human) B3KNQ6, (Bovine) P81948, (Mouse) P68369, (Rat) P68370, (Chinese hamster) P68362, (Chinese hamster) P68361, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Chinese hamster) P68365, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Fruit fly) P06603