Invitrogen

alpha Tubulin Polyclonal Antibody

Product Image Gallery

alpha Tubulin Antibody — Published figure using alpha Tubulin polyclonal antibody (Product # PA5-19489)

alpha Tubulin Antibody in Immunofluorescence (IF) — Immunofluorescent staining of HeLa cells using Product # PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).

alpha Tubulin Antibody in Western Blot (WB) — Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500 µg of lysate with 3 µg of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.01 µg/1x10^6 cells

Immunocytochemistry (ICC)

1-5 µg/mL

Immunofluorescence (IF)

1-5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:2500

Western Blot (WB)

0.5 µg/mL

Published Applications

Immunofluorescence (IF)

See 1 publication below

Western Blot (WB)

See 2 publications below

Immunocytochemistry (ICC)

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Deer, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

RRID

AB_10984311

Product Specific Information

PA5-19489 targets alpha Tubulin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with chicken, Indian muntjac, Chinese hamster, human, fruit fly, rat, African Green Monkey, and mouse samples. Perform heat mediated antigen retrieval via the pressure cooker method before beginning immunohistochemistry staining protocol.

The PA5-19489 immunogen is synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.

PA5-19489 detects alpha Tubulin which has a predicted molecular weight of approximately 50 kDa.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 4; alpha-Tubulin 84B; alpha-Tubulin at 84B; Alpha-tubulin isotype M-alpha-4; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; CG1913-PA; FLJ30169, H2-ALPHA, TUBA1, alpha-tubulin 1; Testis-specific alpha-tubulin; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin H2-alpha; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 4; tubulin, alpha 4a; Tubulin-alpha1; tubulin1alpha; tubulin84B

Gene Aliases: 1t; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; BEST:LD32507; BOS_2117; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; H2-ALPHA; l(3)84Bd; l(3)g3; M[a]4; ms(3)nc33; nc33; T; Talpha1; TBA1_DROME; Tub; TUBA1; Tuba4; TUBA4A; Tuba84B; Tubalpha1; tubulin

UniProt ID: (Human) P68366, (Bovine) P81948, (Rat) Q5XIF6, (Mouse) P68368, (Fruit fly) P06603

Entrez Gene ID: (Dog) 478918, (Human) 7277, (Bovine) 777775, (Rat) 316531, (Chinese hamster) 100767686, (Mouse) 22145, (Fruit fly) 40848

Molecular Function: cytoskeletal protein microtubule family cytoskeletal protein tubulin