Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.5 µg/mL | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
1-5 µg/mL | View 5 publications 5 publications |
Flow Cytometry (Flow) |
0.01 µg/1x10^6 cells | - |
Product Specifications | |
---|---|
Species Reactivity |
Bovine, Chicken, Deer, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.4 |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C or -80°C if preferred |
RRID |
AB_10984311 |
PA5-19489 targets alpha Tubulin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with chicken, Indian muntjac, Chinese hamster, human, fruit fly, rat, African Green Monkey, and mouse samples. Perform heat mediated antigen retrieval via the pressure cooker method before beginning immunohistochemistry staining protocol.
The PA5-19489 immunogen is synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
PA5-19489 detects alpha Tubulin which has a predicted molecular weight of approximately 50 kDa.
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: &agr-tubulin 84B; alpha tubulin; alpha tubulin 84B; alpha-Tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; alpha-Tubulin 84B; Alpha-tubulin I; Alpha-tubulin II; Alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; alpha-tubulin84B; alpha1 tubulin; alpha1-tubulin; alpha[[1]] tubulin; alpha[[1]]-tubulin; alphaT; alphaTub84B-PA; alphatubulin; alphaTubulin 84B; alphaTubulin84B; AT25469p; CG1913-PA; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; Tubulin alpha; tubulin alpha 1c; tubulin alpha 6; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; Tubulin alpha-6 chain; tubulin alpha-8 chain; Tubulin alpha-ubiquitous chain; tubulin alpha1; tubulin alpha1 promoter; Tubulin B-alpha-1; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; Tubulin-alpha1; tubulin1alpha; tubulin84B; unnamed protein product
Gene Aliases: 1t; a-tub84B; a1t; ALPHA 84C; alpha-Tub; alpha-Tub84B; alpha-tubulin; alpha-tubulin 84B; Alpha-Tubulin84B; alpha1; alpha1t; alpha1tub; alpha1tub84B; alpha84B; alphaT; alphat-1; alphaTUB; alphaTub1; alphaTub84; alphaTUB84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; aTub84B; B-ALPHA-1; bA408E5.3; bcm948; BEST:LD32507; CG1913; chr3R:2914276..2914416; clone 1.59; clone 2.30; DM1alpha; Dmel\CG1913; Dmel_CG1913; DTA1; H2-ALPHA; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; LIS3; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; T; Talpha1; TBA1_DROME; Tub; Tub 84B; Tub1C; Tub84B; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; Tuba84B; Tubalpha1; tubulin
UniProt ID: (Human) Q71U36, (Bovine) P81947, (Human) P68363, (Bovine) Q3ZCJ7, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Bovine) Q32KN8, (Human) B3KNQ6, (Bovine) P81948, (Mouse) P68369, (Rat) P68370, (Chinese hamster) P68362, (Chinese hamster) P68361, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Chinese hamster) P68365, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Fruit fly) P06603
Entrez Gene ID: (Chicken) 100857858, (Human) 7846, (Bovine) 404053, (Chicken) 429035, (Bovine) 539882, (Human) 10376, (Bovine) 504244, (Human) 84790, (Human) 7278, (Human) 112714, (Bovine) 534900, (Chicken) 100857247, (Human) 7277, (Bovine) 777775, (Chicken) 421169, (Mouse) 22142, (Rat) 64158, (Chinese hamster) 100682527, (Chinese hamster) 100682528, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Chinese hamster) 100682526, (Rat) 300218, (Chinese hamster) 100767686, (Mouse) 22145, (Rat) 316531, (Fruit fly) 40848
Molecular Function:
tubulin
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