Description: The monoclonal antibody DM1a recognizes the 50 kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).
Applications Reported: This DM1A antibody has been reported for use in immunocytochemistry, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This DM1A antibody has been tested by western blotting under reducing conditions (at 2 µg/mL) or by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha-tubulin 1; Alpha-tubulin 3; Alpha-tubulin isotype M-alpha-1; Detyrosinated tubulin alpha-1A chain; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis specific alpha tubulin; TUBA1; Tubulin alpha; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-alpha-1; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific
Gene Aliases: B-ALPHA-1; LIS3; Tuba-1; Tuba1; TUBA1A; TUBA3