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Invitrogen
c-Myc Recombinant Superclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
c-Myc Antibody (710007) in ICC/IF
Immunofluorescent analysis of c-Myc was performed on 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0. 25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with c-Myc Recombinant Rabbit Polyclonal Antibody (Product # 710007) at a dilution of 1:400 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG secondary antibody (Product # A-11008) at a diluti... View More
Product Details
710007
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to amino acids 1-164, 401-439.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532520
Product Specific Information
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; Class E basic helix-loop-helix protein 39; cmyc; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; Proto-oncogene c-Myc; Transcription factor p64; v-myc myelocytomatosis viral oncogene homolog
Gene Aliases: BHLHE39; c-Myc; MRTL; MYC; MYCC
UniProt ID: (Human) P01106
Entrez Gene ID: (Human) 4609
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein complex binding
protein dimerization activity
repressing transcription factor binding
E-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
branching involved in ureteric bud morphogenesis
positive regulation of mesenchymal cell proliferation
energy reserve metabolic process
chromatin remodeling
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
cellular response to DNA damage stimulus
cell cycle arrest
Notch signaling pathway
positive regulation of cell proliferation
response to gamma radiation
regulation of gene expression
oxygen transport
regulation of telomere maintenance
negative regulation of stress-activated MAPK cascade
cellular response to UV
cellular response to drug
response to drug
negative regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
fibroblast apoptotic process
negative regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
negative regulation of fibroblast proliferation
positive regulation of epithelial cell proliferation
chromosome organization
negative regulation of cell division
canonical Wnt signaling pathway
response to growth factor
positive regulation of metanephric cap mesenchymal cell proliferation
beta-catenin-TCF complex assembly
positive regulation of DNA biosynthetic process
positive regulation of response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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