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Immunofluorescent staining of HeLa using PA5-19601, anti-eIF2B epsilon antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human eIF2B epsilon.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19601 targets eIF2B epsilon in IF and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-19601 immunogen is synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human eIF2B epsilon.
PA5-19601 detects eIF2B epsilon which has a predicted molecular weight of approximately 75 kDa.
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B , a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CACH, CLE, EIF-2B, EIF2Bepsilon, LVWM, eIF-2B GDP-GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; initiation factor eIF-2Be; translation initiation factor eIF-2B subunit epsilon
C81315; CACH; CLE; EIF-2B; EIF2B5; EIF2BE; EIF2Bepsilon; LVWM