Invitrogen

eIF3b Recombinant Rabbit Monoclonal Antibody (JE54-00)

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eIF3b Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of eIF3b in MG-63 cells using a eIF3b monoclonal antibody (Product #MA5-36159). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

eIF3b Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of eIF3b in paraffin-embedded rat cerebellum tissue using a monoclonal antibody (Product #MA5-36159). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

eIF3b Antibody in Western Blot (WB) — Western blot analysis of eIF3b using a eIF3b monoclonal antibody (Product #MA5-36159). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate, Lane 2: 293T cell lysate

eIF3b Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of eIF3b using MCF-7 cells and a eIF3b monoclonal antibody (Product #MA5-36159). The cells were fixed, permeabilized and stained with the primary antibody at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-36159

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JE54-00
Immunogen	Recombinant protein within Human eIF3B aa 81-220 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2890441

Target Information

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Establishing a Prognostic Model Based on Ulceration and Immune Related Genes in Melanoma Patients and Identification of EIF3B as a Therapeutic Target.

Frontiers in immunology

Wu Z,Lei K,Xu S,He J,Shi E

Published figure using eIF3b recombinant monoclonal antibody (Product # MA5-36159) in Western Blot

Mon May 02 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: eIF-3-eta; eIF-3-eta {ECO:0000255|HAMAP-Rule:MF_03001}; eIF3 p110; eIF3 p116; EIF3B; eIF3b {ECO:0000255|HAMAP-Rule:MF_03001}; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit 9 {ECO:0000255|HAMAP-Rule:MF_03001}; Eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001}; eukaryotic translation initiation factor 3, subunit 9 (eta); eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3, subunit B; hPrt1; MGC104664; MGC131875; Prt1 homolog

Gene Aliases: AL033316; AL033334; AL033369; AW208965; D5Wsu45e; EIF3-ETA; EIF3-P110; EIF3-P116; EIF3B; EIF3S9; PRT1

UniProt ID: (Human) P55884, (Mouse) Q8JZQ9, (Rat) Q4G061

Entrez Gene ID: (Human) 8662, (Mouse) 27979, (Rat) 288516

Molecular Function: translation initiation factor