|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 199 and 434 of ENO1 (Uniprot ID#P06733)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-21387 targets enolase 1 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-21387 immunogen is a recombinant fragment corresponding to a region within amino acids 199 and 434 of ENO1 (Uniprot ID#P06733).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells.
PA5-21387 was used in western blot to study the effects of aged garlic extract and its FruArg component by proteomic analysis and lipopolysaccharide-induced neuroinflammatory response in microglial cells
|Zhou H,Qu Z,Mossine VV,Nknolise DL,Li J,Chen Z,Cheng J,Greenlief CM,Mawhinney TP,Brown PN,Fritsche KL,Hannink M,Lubahn DB,Sun GY,Gu Z||PloS one (9:null)||2014|