Immunofluorescent analysis of fumarate hydratase showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a fumarate hydratase polyclonal antibody (Product # PA5-22064) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 195 and 473 of fumarate hydratase (Uniprot ID#P07954)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22064 targets fumarate hydratase in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22064 immunogen is a recombinant fragment corresponding to a region within amino acids 195 and 473 of fumarate hydratase (Uniprot ID#P07954).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.