Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
hnRNP-K Antibody (A300-676A) in IHC
Detection of human and mouse hnRNP-K by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-K (Cat. No. A300-676A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:1,000 (0.2µg/ml). Detection: DAB.
Product Details
A300-676A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 300 and 350 of human Heterogeneous Nuclear Ribonucleoprotein K.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, Rabbit and Orangutan
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progression. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: dC-stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; RP11-575L7.1; Transformation up-regulated nuclear protein; transformation upregulated nuclear protein; TUNP
Gene Aliases: AUKS; CSBP; HNRNPK; HNRPK; KBBP; NOVA; TUNP
UniProt ID: (Human) P61978, (Mouse) P61979
Entrez Gene ID: (Human) 3190, (Mouse) 15387
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
single-stranded DNA binding
RNA binding
mRNA 3'-UTR binding
protein binding
lamin binding
transcription factor binding
protein domain specific binding
pre-mRNA 3'-splice site binding
heat shock protein binding
actinin binding
ribonucleoprotein complex binding
poly(A) RNA binding
ATPase binding
cadherin binding involved in cell-cell adhesion
mRNA CDS binding
C-rich single-stranded DNA binding
core promoter proximal region DNA binding
nucleic acid binding
DNA binding
RNA metabolism protein
negative regulation of transcription from RNA polymerase II promoter
mRNA splicing, via spliceosome
ovarian follicle development
kidney development
liver development
transcription from RNA polymerase II promoter
RNA processing
acute-phase response
signal transduction
peripheral nervous system development
aging
gene expression
regulation of low-density lipoprotein particle clearance
response to activity
viral process
protein sumoylation
cerebellum development
hippocampus development
cerebral cortex development
lung development
negative regulation of protein binding
cellular response to insulin stimulus
positive regulation of RNA splicing
camera-type eye development
negative regulation of apoptotic process
positive regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of mRNA splicing, via spliceosome
positive regulation of receptor-mediated endocytosis
thymus development
positive regulation of synaptic transmission
positive regulation of dendritic spine development
cellular response to amino acid stimulus
cellular response to glucose stimulus
regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter
cellular response to rapamycin
positive regulation of synapse maturation
cell-cell adhesion
positive regulation of long-term synaptic potentiation
response to salt
regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of dendrite extension
cellular response to forskolin
positive regulation of protein localization to cell surface
negative regulation of branching morphogenesis of a nerve
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
mRNA processing
RNA splicing
positive regulation of neuron projection development
cellular response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support