|Tested species reactivity||Amphibian, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human hnRNP proteins from HeLa cells, purified by affinity chromatography on ssDNA agarose.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26745 detects hnRNP L from human, mouse, and newt samples.
MA1-26745 has been successfully used in immunoprecipitation, and Western blot procedures. A band of ~65 kDa can be detected.
The MA1-26745 immunogen is human hnRNP proteins from HeLa cells, purified by affinity chromatography on ssDNA agarose.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid Freeze/Thaw Cycles.
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.