Immunofluorescent analysis of mu Opioid Receptor using a mu Opioid Receptor polyclonal antibody (Product # PA1-24628). The image shows the presence of a subpopulation of spinal lamina II neurons that contained both µ opioid receptor (green) and TRPV1 (red) immunoreactivity in a vehicle (A)- and a RTX (B)-treated rats. Co-localization of the µ opioid receptor and TRPV1 immunoreactivity is indicated in yellow when 2 images are digitally merged. All images are single confocal optical sections. Scale bar: 20 µm.
|Tested species reactivity||Bovine, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues N(384) H Q L E N L E A E T A P L P(398) of rat mu Opioid Receptor.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:2500-1:5000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24628 detects mu Opioid Receptor from human, mouse, rat, and non-human primate samples. PA1-24628 is expected to cross react with bovine (93% conserved), guinea pig (100% conserved), Macaque monkey (100% conserved), and porcine (100% conserved) due to sequence homology.
PA1-24628 has been successfully used in immunohistochemistry (frozen), immunocytochemistry, immunofluorescence and Western blot procedures. The molecular weight of the protein detected by PA1-24628 is 65 kDa.
The PA1-24628 immunogen is a synthetic peptide corresponding to the C-terminal residues N(384) H Q L E N L E A E T A P L P(398) of rat mu Opioid Receptor.
The opioid receptors are G-protein coupled, seven-transmembrane domain receptors for enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa , delta, and mu) were first identified by their different selectivities for various naturally occurring alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid sequences of the opioid receptor subtypes are ~70% homologous, and are similar to somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is thought to occur at the third intracellular loop of the opioid receptors, which is also the location of consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the specific receptor subtypes are found on different chromosomes in both the human and mouse genomes.
The mu opioid receptor is widely distributed throughout the central nervous system, with higher levels found in the periaqueductal gray region and in the superficial dorsal horn of the spinal cord. The mu receptor demonstrates high affinity to enkephalins and beta-endorphin, and low affinity to dynorphins. The mu opioid receptor is also the high affinity binding protein for the opiate alkaloids such as morphine, codeine, and methadone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.