Immunohistochemistry analysis of nNOS showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- nNOS Monoclonal Antibody (3G6B10) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein derived from the N-terminal region of rat nNOS protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding omain. bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. Increases in intracellular calcium lead to the production of low levels of NO over a short time. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intracallosal neuronal nitric oxide synthase neurons colocalize with neurokinin 1 substance P receptor in the rat.
37-2800 was used in immunohistochemistry to examine the localization of neurokinin 1 substance P receptor in rat nitric oxide synthase neurons
|Barbaresi P,Mensà E,Lariccia V,Desiato G,Fabri M,Gratteri S||The Journal of comparative neurology (523:589)||2015|
Differential distribution of parvalbumin- and calbindin-D28K-immunoreactive neurons in the rat periaqueductal gray matter and their colocalization with enzymes producing nitric oxide.
37-2800 was used in western blot to study the distribution, colocalization with enzymes producing nitric oxide, and the synaptic organization of neurons containing parvalbumin and calbindin-D28K in the rat periaqueductal gray matter.
|Barbaresi P,Mensà E,Lariccia V,Pugnaloni A,Amoroso S,Fabri M||Brain research bulletin (99:48)||2013|
Correlation between hippocampal levels of neural, epithelial and inducible NOS and spatial learning skills in rats.
37-2800 was used in western blot to elucidate the role of different nitric oxide synthase isoforms in hippocampus-dependent forms of learning.
|Gökçek-Saraç Ç,Karakurt S,Adal¿ O,Jakubowska-Do¿ru E||Behavioural brain research (235:326)||2012|
Altered sodium channel-protein associations in critical illness myopathy.
37-2800 was used in western blot to study altered sodium channel-protein associated with myopathy.
|Kraner SD,Novak KR,Wang Q,Peng J,Rich MM||Skeletal muscle (2:null)||2012|
Immunocytochemical localization of calretinin-containing neurons in the rat periaqueductal gray and colocalization with enzymes producing nitric oxide: a double, double-labeling study.
37-2800 was used in western blot to determine the localization of calcium-binding protein calretinin and of enzymes producing nitric oxide in the rat periaqueductal gray matter.
|Barbaresi P,Quaranta A,Amoroso S,Mensà E,Fabri M||Synapse (New York, N.Y.) (66:291)||2012|
Functional evidence for nitric oxide production by skeletal-muscle mitochondria from lipopolysaccharide-treated mice.
37-2800 was used in western blot to test if there is a mitochondrially localized nitric oxide synthase.
|Aguirre E,López-Bernardo E,Cadenas S||Mitochondrion (12:126)||2012|
|Human||Not Cited||The nitric oxide synthase family of proteins.||Sessa WC||Journal of vascular research (31:131)||1994|
|Rat||Not Cited||Renal NOS activity, expression, and localization in male and female spontaneously hypertensive rats.||Sullivan JC,Pardieck JL,Hyndman KA,Pollock JS||American journal of physiology. Regulatory, integrative and comparative physiology (298:R61)||2010|
||The nitric oxide synthase family of proteins.||Sessa WC||Journal of vascular research (31:131)||1994|