Invitrogen

p21 Monoclonal Antibody (HJ21)

Advanced Verification

IP-mass spectrometry

10 Published Figures

13 References

View all (83) p21 antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

FIGURE: 1 / 12

p21 Antibody (AHZ0422) in WB

Western blot analysis of p21/Cip1 in PC12 cells using a p21/Cip1 monoclonal antibody (Product # AHZ0422).

p21 Antibody in Immunocytochemistry (ICC/IF)

p21 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

p21 Antibody — IP-MS enrichment of P21 (LFQ intensity): P21 was enriched 75-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and P21 antibody (Product # AHZ0422). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

AHZ0422

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HJ21

Immunogen

Human recombinant p21WAF1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536378

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Senolytic therapy is neuroprotective and improves functional outcome long-term after traumatic brain injury in mice.

Frontiers in neuroscience

Wang J,Lu Y,Carr C,Dhandapani KM,Brann DW

AHZ0422 was used in Immunohistochemistry (Frozen) to indicate there is robust and widespread induction of senescent cells in the brain long-term after TBI, and that senolytic drug treatment begun 1-month after TBI can efficiently ablate the senescent cells, reduce expression of proinflammatory SASP factors, reduce neurodegeneration, and rescue defects in reference memory, recognition memory, and depressive behavior.

Wed Dec 06 00:00:00 EST 2023

Hypoxia-inducible factor-2α mediates senescence-associated intrinsic mechanisms of age-related bone loss.

Experimental & molecular medicine

Lee SY,Park KH,Lee G,Kim SJ,Song WH,Kwon SH,Koh JT,Huh YH,Ryu JH

Published figure using p21 monoclonal antibody (Product # AHZ0422) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Proximal Tubule p53 in Cold Storage/Transplantation-Associated Kidney Injury and Renal Graft Dysfunction.

Frontiers in medicine

Xiang X,Zhu J,Zhang G,Ma Z,Livingston MJ,Dong Z

Published figure using p21 monoclonal antibody (Product # AHZ0422) in Western Blot

Wed Nov 10 00:00:00 EST 2021

A pro longevity role for cellular senescence.

GeroScience

Attaallah A,Lenzi M,Marchionni S,Bincoletto G,Cocchi V,Croco E,Hrelia P,Hrelia S,Sell C,Lorenzini A

AHZ0422 was used in Immunocytochemistry-immunoflourescence to show that cultured cells derived from long-lived species have a higher propensity to undergo senescence when challenged with DNA damage than cells derived from short-lived species.

Mon Jun 01 00:00:00 EDT 2020

Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging.

Oxidative medicine and cellular longevity

Häseli S,Deubel S,Jung T,Grune T,Ott C

Published figure using p21 monoclonal antibody (Product # AHZ0422) in Immunocytochemistry

Thu Feb 04 00:00:00 EST 2021

Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging.

Oxidative medicine and cellular longevity

Häseli S,Deubel S,Jung T,Grune T,Ott C

Published figure using p21 monoclonal antibody (Product # AHZ0422) in Immunocytochemistry

Thu Feb 04 00:00:00 EST 2021

UHPLC-Q/Orbitrap/MS/MS fingerprinting and antitumoral effects of Prosopis strombulifera (LAM.) BENTH. queous extract on allograft colorectal and melanoma cancer models.

Heliyon

Persia FA,Troncoso ME,Rinaldini E,Simirgiotis M,Tapia A,Bórquez J,Mackern-Oberti JP,Hapon MB,Gamarra-Luques C

AHZ0422 was used in Western Blotting to conclude that PsAE is composed by phenolic compounds which demonstrate cytotoxic and antitumoral properties when is orally administrated.

Sat Feb 01 00:00:00 EST 2020

Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells.

Oncology reports

Ohmine S,Salisbury JL,Ingle J,Pettinato G,Haddox CL,Haddad T,Galanis E,Ikeda Y,D'assoro AB

AHZ0422 was used in Western Blot to propose that Aurora-A-targeted therapy could represent a promising prophylactic therapeutic strategy to decrease the likelihood of CIN and development of aggressive teratomas derived from hiPSCs.

Sun Apr 01 00:00:00 EDT 2018

G1 checkpoint is compromised in mouse ESCs due to functional uncoupling of p53-p21Waf1 signaling.

Cell cycle (Georgetown, Tex.)

Suvorova II,Grigorash BB,Chuykin IA,Pospelova TV,Pospelov VA

Published figure using p21 monoclonal antibody (Product # AHZ0422) in Western Blot

Thu Oct 13 00:00:00 EDT 2016

Rapamycin induces pluripotent genes associated with avoidance of replicative senescence.

Cell cycle (Georgetown, Tex.)

Pospelova TV,Bykova TV,Zubova SG,Katolikova NV,Yartzeva NM,Pospelov VA

AHZ0422 was used in immunocytochemistry and western blot to suggest that suppression mTORC activity prevents replicative senescence without transformation of rodent cells.

Sun Dec 15 00:00:00 EST 2013

Rapamycin induces pluripotent genes associated with avoidance of replicative senescence.

Cell cycle (Georgetown, Tex.)

Pospelova TV,Bykova TV,Zubova SG,Katolikova NV,Yartzeva NM,Pospelov VA

AHZ0422 was used in immunocytochemistry and western blot to suggest that suppression mTORC activity prevents replicative senescence without transformation of rodent cells.

Sun Dec 15 00:00:00 EST 2013

p21Waf1 is required for complete oncogenic transformation of mouse embryo fibroblasts by E1Aad5 and c-Ha-ras oncogenes.

Biochimie

Romanov VS,Bardin AA,Zubova SG,Bykova TV,Pospelov VA,Pospelova TV

AHZ0422 was used in western blot to determine the role of p21(Waf1) in the transformation process using mouse embryo fibroblasts.

Thu Sep 01 00:00:00 EDT 2011

Histone deacetylase inhibitor vorinostat suppresses the growth of uterine sarcomas in vitro and in vivo.

Molecular cancer

Hrzenjak A,Moinfar F,Kremser ML,Strohmeier B,Petru E,Zatloukal K,Denk H

Thu Mar 04 00:00:00 EST 2010

Histone deacetylase inhibitor vorinostat suppresses the growth of uterine sarcomas in vitro and in vivo.

Molecular cancer

Hrzenjak A,Moinfar F,Kremser ML,Strohmeier B,Petru E,Zatloukal K,Denk H

Thu Mar 04 00:00:00 EST 2010

Pluripotent cell division cycles are driven by ectopic Cdk2, cyclin A/E and E2F activities.

Oncogene

Stead E,White J,Faast R,Conn S,Goldstone S,Rathjen J,Dhingra U,Rathjen P,Walker D,Dalton S

AHZ0422 was used in Western Blotting to show that rapid cell division cycles in primitive cells of embryonic origin are driven by extreme levels of Cdk activity that lack normal cell cycle periodicity.

Thu Nov 28 00:00:00 EST 2002

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p21 Monoclonal Antibody (HJ21)

p21 Antibody (AHZ0422) in WB

Product Details

AHZ0422

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Target Information

Performance Guarantee

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