|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
|Western Blot (WB)||See 4 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Species reactivity||Human, Mouse, Rat|
|Published species||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant p21WAF1 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDNK1A; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; p21; p21<WAF1/CIP1>; p21CIP1; SDI1; WAF1; wild-type p53-activated fragment 1
Gene Aliases: CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; UV96; WAF1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.