|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant p21WAF1 protein.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:20|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.
IP-MS enrichment of P21 (LFQ intensity): P21 was enriched 75-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and P21 antibody (Part No. AHZ0422). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
G1 checkpoint is compromised in mouse ESCs due to functional uncoupling of p53-p21Waf1 signaling.
AHZ0422 was used in western blot to determine the role of uncoupling of p53/p21-Waf1 signaling and G1 checkpoint disruption in mouse ESCs
|Suvorova II,Grigorash BB,Chuykin IA,Pospelova TV,Pospelov VA||Cell cycle (Georgetown, Tex.) (15:52)||2016|
p21Waf1 is required for complete oncogenic transformation of mouse embryo fibroblasts by E1Aad5 and c-Ha-ras oncogenes.
AHZ0422 was used in western blot to determine the role of p21(Waf1) in the transformation process using mouse embryo fibroblasts.
|Romanov VS,Bardin AA,Zubova SG,Bykova TV,Pospelov VA,Pospelova TV||Biochimie (93:1408)||2011|
|Histone deacetylase inhibitor vorinostat suppresses the growth of uterine sarcomas in vitro and in vivo.||Hrzenjak A,Moinfar F,Kremser ML,Strohmeier B,Petru E,Zatloukal K,Denk H||Molecular cancer (9:null)||2010|
Rapamycin induces pluripotent genes associated with avoidance of replicative senescence.
AHZ0422 was used in immunocytochemistry and western blot to suggest that suppression mTORC activity prevents replicative senescence without transformation of rodent cells.
|Pospelova TV,Bykova TV,Zubova SG,Katolikova NV,Yartzeva NM,Pospelov VA||Cell cycle (Georgetown, Tex.) (12:3841)||2013|