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Immunofluorescence analysis of p73 alpha/beta was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p73 alpha/beta Antibody (ER-15) Mouse Monoclonal Antibody (MA514117) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified monkey recombinant p73alpha protein (aa380-637)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14117 targets p73alpha/beta in IP and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-14117 immunogen is purified monkey recombinant p73alpha protein (aa380-637).
p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. p73 is expressed in either a full-length form (alpha) (MW ~80kDa) or a shorter (beta) (MW ~70kDa) mRNA variant, with exon 13 spliced out. Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53-dependent cell cycle arrest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The differential effects of wild-type and mutated K-Ras on MST2 signaling are determined by K-Ras activation kinetics.
MA5-14117 was used in western blot to study the role of altered K-Ras activation kinetics in the differential effects of wild-type and mutant K-Ras on MST2 pathways
|Romano D,Maccario H,Doherty C,Quinn NP,Kolch W,Matallanas D||Molecular and cellular biology (33:1859)||2013|
TAp73ß-mediated suppression of cell migration requires p57Kip2 control of actin cytoskeleton dynamics.
MA5-14117 was used in western blot to study the role of p57Kip2-mediated modulation of the cytoskeleton in the mechanism by which TAp73-beta inhibits cell migration
|Rodhe J,Kavanagh E,Joseph B||Oncotarget (4:289)||2013|
IkappaB kinase beta promotes cell survival by antagonizing p53 functions through DeltaNp73alpha phosphorylation and stabilization.
MA5-14117 was used in western blot to study the role of IKKbeta-mediated phosphorylation and stabilization of deltaNp73alpha in antagonizing p53 function and enhancing cell survival
|Accardi R,Scalise M,Gheit T,Hussain I,Yue J,Carreira C,Collino A,Indiveri C,Gissmann L,Sylla BS,Tommasino M||Molecular and cellular biology (31:2210)||2011|
PML, YAP, and p73 are components of a proapoptotic autoregulatory feedback loop.
MA5-14117 was used in western blot to study the role of PML, YAP, and p73 in the regulation of apoptotic process
|Lapi E,Di Agostino S,Donzelli S,Gal H,Domany E,Rechavi G,Pandolfi PP,Givol D,Strano S,Lu X,Blandino G||Molecular cell (32:803)||2008|
Resistance of mitochondrial p53 to dominant inhibition.
MA5-14117 was used in western blot to examine the dominant inhibition of p53 mutation
|Heyne K,Schmitt K,Mueller D,Armbruester V,Mestres P,Roemer K||Molecular cancer (7:null)||2008|
Sp1-like sequences mediate human caspase-3 promoter activation by p73 and cisplatin.
MA5-14117 was used in western blot to study the role of Sp1-like sequences in human caspase-3 promoter activation by p73 and cisplatin
|Sudhakar C,Jain N,Swarup G||The FEBS journal (275:2200)||2008|
p53 dependent and independent sensitivity to oxaliplatin of colon cancer cells.
MA5-14117 was used in western blot to study the p53-dependent and -independent sensitivity to oxaliplatin of colon cancer cells
|Toscano F,Parmentier B,Fajoui ZE,Estornes Y,Chayvialle JA,Saurin JC,Abello J||Biochemical pharmacology (74:392)||2007|
MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells.
MA5-14117 was used in western blot to study the role of MDM2 in the suppression of apoptosis by activated Akt1 in salivary acinar cells
|Limesand KH,Schwertfeger KL,Anderson SM||Molecular and cellular biology (26:8840)||2006|
S100A2 gene is a direct transcriptional target of p53 homologues during keratinocyte differentiation.
MA5-14117 was used in western blot to investigate the regulatory effects of p53 homologues on S100A2 transcription during keratinocyte differentiation
|Lapi E,Iovino A,Fontemaggi G,Soliera AR,Iacovelli S,Sacchi A,Rechavi G,Givol D,Blandino G,Strano S||Oncogene (25:3628)||2006|
Over-expression of p73beta results in apoptotic death of post-mitotic hNT neurons.
MA5-14117 was used in western blot to study the role of p73beta overexpression in apoptotic death of post-mitotic hNT neurons
|Jiang Y,Lo W,Akhmametyeva EM,Chang LS||Journal of the neurological sciences (240:1)||2006|
New antibodies recognizing p73: comparison with commercial antibodies.
MA5-14117 was used in western blot to compare three new anti-p73 antibodies with those commercially available
|Sayan AE,Paradisi A,Vojtesek B,Knight RA,Melino G,Candi E||Biochemical and biophysical research communications (330:186)||2005|
ASPP1 and ASPP2: common activators of p53 family members.
MA5-14117 was used in western blot to study the role of ASPP1 and ASPP2 in the p53 signaling pathway
|Bergamaschi D,Samuels Y,Jin B,Duraisingham S,Crook T,Lu X||Molecular and cellular biology (24:1341)||2004|
Exclusion of c-Abl from the nucleus restrains the p73 tumor suppression function.
MA5-14117 was used in western blot to investigate the effect of c-Abl nuclear exclusion on p73 function in human cancers
|Vella V,Zhu J,Frasca F,Li CY,Vigneri P,Vigneri R,Wang JY||The Journal of biological chemistry (278:25151)||2003|
Identification and characterization of HIPK2 interacting with p73 and modulating functions of the p53 family in vivo.
MA5-14117 was used in western blot to study the interaction of HIPK2 with p73 and other p53 family members and its potential role in regulating apoptosis and the cell cycle
|Kim EJ,Park JS,Um SJ||The Journal of biological chemistry (277:32020)||2002|
Isoform-specific p73 knockout mice reveal a novel role for delta Np73 in the DNA damage response pathway.
MA5-14117 was used in immunocytochemistry to investigate the effect of delta Np73 on the induction of DNA damage response in vivo
|Wilhelm MT,Rufini A,Wetzel MK,Tsuchihara K,Inoue S,Tomasini R,Itie-Youten A,Wakeham A,Arsenian-Henriksson M,Melino G,Kaplan DR,Miller FD,Mak TW||Genes and development (24:549)||2010|
Zic deficiency in the cortical marginal zone and meninges results in cortical lamination defects resembling those in type II lissencephaly.
MA5-14117 was used in immunohistochemistry to detect the role of Zic in cortical lamination and its implication in type II lissencephaly
|Inoue T,Ogawa M,Mikoshiba K,Aruga J||The Journal of neuroscience : the official journal of the Society for Neuroscience (28:4712)||2008|
p73 expression in medulloblastoma: TAp73/DeltaNp73 transcript detection and possible association of p73alpha/DeltaNp73 immunoreactivity with survival.
MA5-14117 was used in immunohistochemistry to investigate the expression of p73 in medulloblastoma specimens
|Zitterbart K,Zavrelova I,Kadlecova J,Spesna R,Kratochvilova A,Pavelka Z,Sterba J||Acta neuropathologica (114:641)||2007|
Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes.
MA5-14117 was used in immunohistochemistry to study the mechanism underlying the different proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes
|Lee J,Basak JM,Demehri S,Kopan R||Development (Cambridge, England) (134:2795)||2007|
Massive loss of Cajal-Retzius cells does not disrupt neocortical layer order.
MA5-14117 was used in immunohistochemistry to investigate the influence of Cajal-Retzius cell reduction on the development of neocortical layer order
|Yoshida M,Assimacopoulos S,Jones KR,Grove EA||Development (Cambridge, England) (133:537)||2006|
Developmental roles of p73 in Cajal-Retzius cells and cortical patterning.
MA5-14117 was used in immunohistochemistry to study the role of p73 in brain development
|Meyer G,Cabrera Socorro A,Perez Garcia CG,Martinez Millan L,Walker N,Caput D||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:9878)||2004|
p73 Overexpression and angiogenesis in human colorectal carcinoma.
MA5-14117 was used in immunohistochemistry to study the expression of p73 in colorectal cancer and its potential role in tumor angiogenesis
|Guan M,Peng HX,Yu B,Lu Y||Japanese journal of clinical oncology (33:215)||2003|
p73 tumor-suppressor activity is impaired in human thyroid cancer.
MA5-14117 was used in immunoprecipitation to study the p73 tumor-suppressor activity in human thyroid cancer
|Frasca F,Vella V,Aloisi A,Mandarino A,Mazzon E,Vigneri R,Vigneri P||Cancer research (63:5829)||2003|
p53-like transcription factor; p53-related protein; Tumor protein p73