Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:5,000 | View 2 publications 2 publications |
Immunohistochemistry (Frozen) (IHC (F)) |
1:1000-1:5000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to residues (C-E(69) L L D E D Q K V R P N E E(82) of human BDNF precursor protein conjugated to KLH. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
Conc. Not Determined |
Storage buffer |
whole serum |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_1086805 |
PA1-18360 detects proBDNF from human, mouse, and rat samples. This antibody is expected to react with most mammals due to sequence homology.
PA1-18360 has been successfully used in immunohistochemistry and Western blot procedures. In Western blot, this anitserum detects a 35 kDa band corresponding to the molecular weight of proBDNF.
The PA1-18360 immunogen is a synthetic peptide corresponding to residues (C-E(69) L L D E D Q K V R P N E E(82) of human BDNF precursor, conjugated to KLH.
Reconstitute with 100 µL of distilled water. Glycerol (1:1) may be added for additional stability.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimers and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Abrineurin; anorexia BDNF; BDNF; BDNF precursor form; brain derived neurothrophic factor; brain derived neurotrophic factor; Brain-derived neurotrophic factor; neurotrophin; pro BDNF; ProBDNF
Gene Aliases: ANON2; BDNF; BULN2
UniProt ID: (Human) P23560, (Rat) P23363, (Mouse) P21237
Entrez Gene ID: (Human) 627, (Rat) 24225, (Mouse) 12064
Molecular Function:
neurotrophic factor
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