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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Recombinant p21 ras protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. These proteins function in signal transduction pathways. They can bind GTP and GDP, and they have intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in the HRAS gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||2.5 µg/ml||Immortalized fibroblasts from NF-kappaB RelA knockout mice show phenotypic heterogeneity and maintain increased sensitivity to tumor necrosis factor alpha after transformation by v-Ras.||Gapuzan ME,Schmah O,Pollock AD,Hoffmann A,Gilmore TD||Oncogene (24:6574)||2005|
|Human||Not Cited||Melanoma cell migration to type IV collagen requires activation of NF-kappaB.||Hodgson L,Henderson AJ,Dong C||Oncogene (22:98)||2003|
c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; GTPase HRas; H-ras 1 protein; H-Ras-1; Ha-Ras1 proto-oncoprotein; Harvey ras1 protein; Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; Harvey-ras; Hras; Hras1; p19 H-RasIDX protein; p21ras; Ras; Ras family small GTP binding protein H-Ras; transformation gene: oncogene HAMSV; transforming protein P21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog
C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; c-rasHa; CTLO; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; Kras2; p21ras; ras; RASH1