Cadherin pan Antibody (717100) in IF

Immunofluorescent detection of pan cadherin in MDCK cells. Confluent monolayers were fixed in 50%methanol/50%Acetone, blocked for at least 30 minutes in 1% BSA then incubated 2 hours with a pan cadherin antibody (Product # 71-7100) at 2.5 ug/mL, washed, then incubated 1 hour with Alexa Flour 594 conjugated Donkey anti-Rabbit secondary antibody (Product # A21207) at 1:2000 dilution. Cells were counterstained with DAPI (blue). Coverslips were mounted with Prolong Gold Antifade reagent (Product # P36930) and imaged at 40X. Images generated by Joell Solan in Paul Lampe Lab at the Fred Hutchinson cancer Research Center.

Immunofluorescent detection of pan cadherin in MDCK cells. Confluent monolayers were fixed in 50%methanol/50%Acetone, blocked for at least 30 minutes in 1% BSA then incubated 2 hours with a pan cadherin antibody (Product # 71-7100) at 2.5 ug/mL, washed, then incubated 1 hour with Alexa Flour 594 conjugated Donkey anti-Rabbit secondary antibody (Product # A21207) at 1:2000 dilution. Cells were counterstained with DAPI (blue). Coverslips were mounted with Prolong Gold Antifade reagent (Product # P36930) and imaged at 40X. Images generated by Joell Solan in Paul Lampe Lab at the Fred Hutchinson cancer Research Center.

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