Animal Health Disease Detection Support—Getting Started
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We recommend running the Xeno Internal Positive Control with our animal health qPCR reagents as a positive control and to help determine whether or not a sample has inhibitors. Many animal health samples will not have a high level or any disease target, so running the Xeno control allows you to be confident that your qPCR reaction worked and that the absence of target signal is due to the true absence of the target instead of a reaction failure. We have Xeno Control Assays labeled with either VIC or LIZ.
We recommend one of the following assays:
- VetMAX Xeno Internal Positive Control—VIC Assay (Cat. No. A29765)
- VetMAX Xeno Internal Positive Control—LIZ Assay (Cat. No. A29766).
The VetMAX Xeno Internal Positive Control is derived from a plasmid source.
The quencher used in these assays is an Eclipse Q quencher. Within the qPCR instrument’s software, choose “none”.
Generally, we recommend using this master mix with standard cycling conditions, but depending on the assay being used, you could use fast cycling conditions with optimization.
Yes, the qPCR master mix is supplied in the Green tube (3-Mix QPCV2).
The TaqMan assay for QPCV2 is labeled with FAM and quenched by NFQ (non-fluorescent quencher). The TaqMan assay for IPC is labeled with VIC and quenched with TAMRA.
The TaqMan assay for TUB is labeled with FAM and quenched by NFQ (non-fluorescent quencher). The TaqMan assay for IPC is labeled with VIC and quenched by NFQ (non-fluorescent quencher).
The master mix used in the VetMAX PEDV/TGEV/SDCoV Kit is the TaqMan Fast Virus 1-Step Master Mix.
The TaqMan assay for PEDV RNA is labeled with LIZ, the TaqMan assay for TGEV is labeled with FAM, the TaqMan assay for SDCoV is labeled with VIC, and the TaqMan assay for the Xeno RNA is labeled with NED. All four TaqMan assays are combined in the VetMAX PEDV/TGEV/SDCoV Primer Probe Mix.
For Veterinary Use Only. For In Vitro Use Only