Two-color infrared Western blot detection using DyLight 680- and DyLight 800-Conjugated Secondary Antibodies

p53 (53 kDa; red) and cyclophilin B (21 kDa; green) levels in response to siRNA expression in A549 cells were detected by Western blot analysis using DyLight 680- (red) and DyLight 800- (green) Conjugated Secondary Antibodies. Beta-catenin (90 kDa, green) was used as the load control. Imaged with the Odyssey™ Infrared Imaging System using the 700 and 800 channels.