ICC comparison of Click-iT® Plus TUNEL with competitor assay

HeLa cells were transduced with CellLight® Mitochondria-GFP, BacMam 2.0 (green) and treated with DNase to induce TUNEL-positive DNA strand breaks. TUNEL-positive cells were identified with either the Roche In situ Cell Death TMR (orange) or Life Technologies– Click-iT® Plus TUNEL assay with Alexa Fluor® 594 dye (red). Actin filaments were stained with phalloidin conjugated with Alexa Fluor® 647 dye (purple).

HeLa cells were transduced with CellLight® Mitochondria-GFP, BacMam 2.0 (green) and treated with DNase to induce TUNEL-positive DNA strand breaks. TUNEL-positive cells were identified with either the Roche In situ Cell Death TMR (orange) or Life Technologies– Click-iT® Plus TUNEL assay with Alexa Fluor® 594 dye (red). Actin filaments were stained with phalloidin conjugated with Alexa Fluor® 647 dye (purple).

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