HA Tag Antibody (PA1-985) in WB

Western blot analysis of 293T/17 whole cell lysate (Lane 1) and HA-Tag transfected 293T/17 cell lysates (Lane 2). Samples were loaded at 20µg per lane onto a 15% SDS-polyacrylamide gel, transferred to a PVDF membrane (semi-dry) and blocked with TBS containing TBST and 5% non-fat dry milk for 2 hrs at room temperature. The membrane was washed in TBST and probed with an anti-HA Tag polyclonal antibody (Product ## PA1-985) at a dilution of 1:5000 overnight at 4ºC and washed in TBST. Secondary detection was performed using a Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, HRP conjugate (Product ## 31462) at a dilution of 1:5000, followed by ECL detection. Data provided courtesy of Antibody Resource.

Western blot analysis of 293T/17 whole cell lysate (Lane 1) and HA-Tag transfected 293T/17 cell lysates (Lane 2). Samples were loaded at 20µg per lane onto a 15% SDS-polyacrylamide gel, transferred to a PVDF membrane (semi-dry) and blocked with TBS containing TBST and 5% non-fat dry milk for 2 hrs at room temperature. The membrane was washed in TBST and probed with an anti-HA Tag polyclonal antibody (Product ## PA1-985) at a dilution of 1:5000 overnight at 4ºC and washed in TBST. Secondary detection was performed using a Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, HRP conjugate (Product ## 31462) at a dilution of 1:5000, followed by ECL detection. Data provided courtesy of Antibody Resource.

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