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クローンが増殖しません。 改善策があるのでしょうか？

We ship clones as glycerol stocks at –80°C. We recommend making a fresh glycerol stock soon after receipt. Here are some additional suggestions:

  • Verify whether the correct antibiotic was used to grow the clone.
    • Check vector map for antibiotic resistance gene or
    • Check cap color of tube containing the glycerol stock and use the information below to determine which antibiotic to use:
      • Yellow cap: Zeocin
      • Purple cap: chloramphenicol
      • Red cap: carbenicillin or ampicillin
      • Green cap: kanamycin
クローンのシーケンシングを行いましたが、シーケンスが正しくありません。 改善策があるのでしょうか？

This may be due to one of the following reasons:

  • Database error – there is a ~12.5% error rate in GenBank for cDNA clones, meaning that the sequence in the GenBank database does not match the sequence of the clone in the indicated well.
  • Incorrect pick in the manufacturing lab – these clones are housed in 96-well (or in the case of BACs, 384-well) plates and human error can occur. If this is the issue, getting a replacement would help. This issue is less likely in case of Ultimate™ ORF clones as these are not stored in plates but in individual tubes.
  • A mixed population in the plate – for the same reason as in #2, these wells can become cross-contaminated. We always recommend analyzing several colonies from any particular culture.

Note: Besides Ultimate™ ORF clones, other clones are not guaranteed to exactly match GenBank sequences and may differ by one or more bases because of allelic and isoform variances.

使用している MGC クローンが NCBI データベース中にありますが、CloneRanger™ ツールではヒットしません。 何が原因と考えられますか？
  • We may have had this clone in the past, but it might have been removed from the collection because it was a “no grow”
  • If it is a recently created clone, it might take us a while to stock it. We only offer clones that have been entered into the NCBI database more than 6 months to 1 year ago.
  • Please check with ATTC ( www.attc.org ) or Open Biosystems (www.openbiosystems.com) to see if they carry the clone .

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For Research Use Only. Not for use in diagnostic procedures.