Galectin 4 Antibody (33-8600) in IF

Immunofluorescent analysis of Gal4 (green) in Gal4 DNA Binding Domain expressing 293T cells (Product # K1091A). The cells were fixed with formalin for 20 minutes, permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Gal4 mouse monoclonal antibody (Product # 33-8600), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Immunofluorescent analysis of Gal4 (green) in Gal4 DNA Binding Domain expressing 293T cells (Product # K1091A). The cells were fixed with formalin for 20 minutes, permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Gal4 mouse monoclonal antibody (Product # 33-8600), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›