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Detection of hypoxic conditions at the core of spheroid using Image-iT® Hypoxia Reagent
HeLa cells were grown on Nunc™ Sphera 96-well U-bottomed plates for 2 days for formation of spheroids in complete medium. The spheroids were stained with 5 µM Image-iT® Hypoxia Probe (red) for 3 hr, and NucBlue® Live (blue) was used as nuclear counter stain. The images were taken on a confocal microscope.
