SLC7A11 Antibody (711589) in IF

For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous xCT/ SLC7A11 using ABfinity? xCT SLC7A11 Recombinant Rabbit Oligoclonal Antibody (Product ## 711589, 2ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, Alexa Fluor® 488 conjugate (Product ## A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of xCT/ SLC7A11 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product ## S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product ## R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of xCT/ SLC7A11 in the membrane/cytoplasm. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

For immunofluorescence analysis, SH-SY5Y cells were fixed and permeabilized for detection of endogenous xCT/ SLC7A11 using ABfinity? xCT SLC7A11 Recombinant Rabbit Oligoclonal Antibody (Product ## 711589, 2ug/ml) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, Alexa Fluor® 488 conjugate (Product ## A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of xCT/ SLC7A11 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product ## S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product ## R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of xCT/ SLC7A11 in the membrane/cytoplasm. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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