Cellular hydrogen peroxide detection in U2-OS cells using Premo™ Cellular Hydrogen Peroxide Sensor

U2-OS cells were plated on 35 mm dishes (MatTek) at a density of 75,000 cells and transduced with Premo™ Cellular Hydrogen Peroxide Sensor. After 48 hrs, time lapse imaging was done on a confocal microscope using 400 nm and 488 nm lasers for excitation with the emission at 515 nm. The images were taken every 15 seconds after addition of various doses of hydrogen peroxide for 10 minutes. Fluorescence intensity values were quantified by making regions of interest on the cells and were used to calculate 400/488 nm excitation ratios. The ratios were plotted against time.

U2-OS cells were plated on 35 mm dishes (MatTek) at a density of 75,000 cells and transduced with Premo™ Cellular Hydrogen Peroxide Sensor. After 48 hrs, time lapse imaging was done on a confocal microscope using 400 nm and 488 nm lasers for excitation with the emission at 515 nm. The images were taken every 15 seconds after addition of various doses of hydrogen peroxide for 10 minutes. Fluorescence intensity values were quantified by making regions of interest on the cells and were used to calculate 400/488 nm excitation ratios. The ratios were plotted against time.

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