Dionex™ CarboPac™ PA100 IC Columns
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Thermo Scientific™

Dionex™ CarboPac™ PA100 IC Columns

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Resolve oligosaccharides released from glycoproteins differing in size, charge, branching, and linkage with Thermo Scientific™ Dionex™ CarboPac™ PA100 Analytical & Guard Columns.
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Catalog NumberColumn TypeLength (Metric)Diameter (Metric)
057182Analytical Column250 mm2 mm
043055Analytical Column250 mm4 mm
043054Guard Column50 mm4 mm
057183Guard Column50 mm2 mm
Catalog number 057182
Price (USD)/ Each 1
1,870.00
Add to cart
Column Type:
Analytical Column
Length (Metric):
250 mm
Diameter (Metric):
2 mm
Price (USD)/ Each 1
1,870.00
Add to cart
Efficiently resolve oligosaccharides released from therapeutic glycoproteins differing in size, charge, branching, and linkage with the Thermo Scientific™ Dionex™ CarboPac™ PA100 Analytical & Guard Columns. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection (PAD), this high-resolution, strong anion-exchange column supports detection at low-picomole levels for enhanced chromatography of oligosaccharides.
The resin of Dionex CarboPac PA100 Columns consists of 5.5μm diameter nonporous beads covered with a fine layer of functionalized Thermo Scientific™ Dionex™ MicroBead™ latex. This pellicular resin structure permits excellent mass transfer, resulting in high-resolution chromatography and rapid re-equilibration. The 3 x 250mm format provides fast separations, and the 0.5mL/minute recommended flow rate results in significant savings in eluent consumption.

Predictable, High-Resolution Separations
  • Separates closely related oligosaccharides, even isomers, at picomole levels
  • Separates neutral and charged oligosaccharides in the same run
  • Requires no sample derivatization for detection

Use the Thermo Scientific™ Dionex™ CMD™ Carbohydrate Membrane Desalter on line prior to fraction collection to provide easy, automated removal of eluent salts. 

About the Dionex CarboPac Family of Columns

The Dionex CarboPac family offers a selection of high-performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides. 

Dionex CarboPac columns provide high-resolution separations of:

  • Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
  • Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates
  • Mono- and disaccharides
Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
Column Capacity22.5 μeq
Column ConstructionPEEK
Flow Rate1.0 mL/min.
For Use With (Application)Carbohydrate Analysis
For Use With (Equipment)HPAE-PAD
Green FeaturesResponsibly packaged
Max. Pressure4000 psi (275 bar)
Particle Size8.5 μm
QuantityEach 1
Resin Composition8.5 μm Diameter Ethylvinylbenzene/Divinylbenzene Substrate (55% Cross-Linking) Agglomerated with 275 nm MicroBead Quaternary Amine Functionalized Latex (6% Cross-Linked).
Solvent Compatibility100% Compatible with Common Organic Solvents; Ionic Form Eluents: Sodium Acetate and Sodium Hydroxide
Stationary PhaseCarboPac PA100
Temperature (Metric)4°C to 60°C
Column TypeAnalytical Column
Diameter (Metric)2 mm
FormatMicrobore
Length (Metric)250 mm
pH0 to 14
Unit SizeEach 1