Thermo Scientific™

pH Gradient Buffers

Catalog number: 083273
Thermo Scientific™

pH Gradient Buffers

Catalog number: 083273

Obtain fast and highly robust, reproducible HPLC gradients using Thermo Scientific™ CX-1 pH Gradient Buffers. Simple to optimize and easily automated, these buffers are applicable for HPLC separation of the majority of monoclonal antibody (mAb) biotherapeutics drugs. CX-1 pH Gradient Buffers are ready to use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a wide range of mAb charge variants through a generic HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.

 
Catalog Number
083273
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
125 mL
Price (USD)
155.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)125 mL
TypeCX-1 pH Gradient Buffer A
DescriptionCX-1 pH Gradient Buffer A
Quantity125 mL
pH5.6
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
125 mL
Price (USD)
155.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)125 mL
TypeCX-1 pH Gradient Buffer B
DescriptionCX-1 pH Gradient Buffer B
Quantity125 mL
pH10.2
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer Kit;
125 mL Buffer A + 125 mL Buffer B
pH
5.6 to 10.2
Quantity
125 mL + 125 mL
Price (USD)
309.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)125 mL
TypeCX-1 pH Gradient Buffer Kit
DescriptionCX-1 pH Gradient Buffer Kit;
125 mL Buffer A + 125 mL Buffer B
Quantity125 mL + 125 mL
pH5.6 to 10.2
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
250 mL
Price (USD)
253.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)250 mL
TypeCX-1 pH Gradient Buffer A
DescriptionCX-1 pH Gradient Buffer A
Quantity250 mL
pH5.6
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
250 mL
Price (USD)
253.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)250 mL
TypeCX-1 pH Gradient Buffer B
DescriptionCX-1 pH Gradient Buffer B
Quantity250 mL
pH10.2
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer Kit;
250 mL Buffer A + 250 mL Buffer B
pH
5.6 to 10.2
Quantity
250 mL + 250 mL
Price (USD)
501.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)250 mL
TypeCX-1 pH Gradient Buffer Kit
DescriptionCX-1 pH Gradient Buffer Kit;
250 mL Buffer A + 250 mL Buffer B
Quantity250 mL + 250 mL
pH5.6 to 10.2
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
500 mL
Price (USD)
430.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
DescriptionCX-1 pH Gradient Buffer A
Quantity500 mL
pH5.6
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
500 mL
Price (USD)
430.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
DescriptionCX-1 pH Gradient Buffer B
Quantity500 mL
pH10.2
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Starter Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (10 µm, 4 x 250 mm)
pH
5.6 to 10.2
Quantity
125 mL + 125 mL
Price (USD)
Availability
***
Full specifications
Quantity
-
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Starter Kit
DescriptionCX-1 pH Gradient Starter Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (10 µm, 4 x 250 mm)
Quantity125 mL + 125 mL
pH5.6 to 10.2
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient High Throughput Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (5 µm, 4 x 50 mm)
pH
5.6 to 10.2
Quantity
125 mL + 125 mL
Price (USD)
Availability
***
Full specifications
Quantity
-
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient High Throughput Kit
DescriptionCX-1 pH Gradient High Throughput Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (5 µm, 4 x 50 mm)
Quantity125 mL + 125 mL
pH5.6 to 10.2
Unit SizeEach
Showing 10 of 13Show more
Catalog NumberSpecificationsUnit SizeDescriptionpHQuantityPrice (USD)AvailabilityQuantity
083273Full specifications
EachCX-1 pH Gradient Buffer A5.6125 mL
155.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)125 mL
TypeCX-1 pH Gradient Buffer A
DescriptionCX-1 pH Gradient Buffer A
Quantity125 mL
pH5.6
Unit SizeEach
083275Full specifications
EachCX-1 pH Gradient Buffer B10.2125 mL
155.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)125 mL
TypeCX-1 pH Gradient Buffer B
DescriptionCX-1 pH Gradient Buffer B
Quantity125 mL
pH10.2
Unit SizeEach
083274Full specifications
EachCX-1 pH Gradient Buffer Kit;
125 mL Buffer A + 125 mL Buffer B
5.6 to 10.2125 mL + 125 mL
309.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)125 mL
TypeCX-1 pH Gradient Buffer Kit
DescriptionCX-1 pH Gradient Buffer Kit;
125 mL Buffer A + 125 mL Buffer B
Quantity125 mL + 125 mL
pH5.6 to 10.2
Unit SizeEach
085346Full specifications
EachCX-1 pH Gradient Buffer A5.6250 mL
253.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)250 mL
TypeCX-1 pH Gradient Buffer A
DescriptionCX-1 pH Gradient Buffer A
Quantity250 mL
pH5.6
Unit SizeEach
085348Full specifications
EachCX-1 pH Gradient Buffer B10.2250 mL
253.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)250 mL
TypeCX-1 pH Gradient Buffer B
DescriptionCX-1 pH Gradient Buffer B
Quantity250 mL
pH10.2
Unit SizeEach
085349Full specifications
EachCX-1 pH Gradient Buffer Kit;
250 mL Buffer A + 250 mL Buffer B
5.6 to 10.2250 mL + 250 mL
501.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
Volume (Metric)250 mL
TypeCX-1 pH Gradient Buffer Kit
DescriptionCX-1 pH Gradient Buffer Kit;
250 mL Buffer A + 250 mL Buffer B
Quantity250 mL + 250 mL
pH5.6 to 10.2
Unit SizeEach
302779Full specifications
EachCX-1 pH Gradient Buffer A5.6500 mL
430.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
DescriptionCX-1 pH Gradient Buffer A
Quantity500 mL
pH5.6
Unit SizeEach
302780Full specifications
EachCX-1 pH Gradient Buffer B10.2500 mL
430.00
Your Price:
***
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
DescriptionCX-1 pH Gradient Buffer B
Quantity500 mL
pH10.2
Unit SizeEach
083381Full specifications
EachCX-1 pH Gradient Starter Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (10 µm, 4 x 250 mm)
5.6 to 10.2125 mL + 125 mLRequest A Quote***-
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Starter Kit
DescriptionCX-1 pH Gradient Starter Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (10 µm, 4 x 250 mm)
Quantity125 mL + 125 mL
pH5.6 to 10.2
Unit SizeEach
083378Full specifications
EachCX-1 pH Gradient High Throughput Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (5 µm, 4 x 50 mm)
5.6 to 10.2125 mL + 125 mLRequest A Quote***-
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient High Throughput Kit
DescriptionCX-1 pH Gradient High Throughput Kit;
125 mL Buffer A + 125 mL Buffer B
MAbPac SCX-10 Column (5 µm, 4 x 50 mm)
Quantity125 mL + 125 mL
pH5.6 to 10.2
Unit SizeEach
Showing 10 of 13Show more

pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.

  • Robust, reproducible gradients
  • Predict biomolecule pH
  • Simple method optimization
  • Easily automated
  • Applicable to the majority of MAbs

Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.

WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

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Frequently asked questions (FAQs)

Citations & References