pH Gradient Buffers
Obtain fast and highly robust, reproducible HPLC gradients using Thermo Scientific™ CX-1 pH Gradient Buffers. Simple to optimize and easily automated, these buffers are applicable for HPLC separation of the majority of monoclonal antibody (mAb) biotherapeutics drugs. CX-1 pH Gradient Buffers are ready to use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a wide range of mAb charge variants through a generic HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.
pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.
- Robust, reproducible gradients
- Predict biomolecule pH
- Simple method optimization
- Easily automated
- Applicable to the majority of MAbs
Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.
Thermo Scientific pH buffer concentrates used in the pH gradient platform can be purchased individually or as a pair, in quantities of 125mL or 250mL. For added convenience, the 125mL buffers can also be bundled with columns in a number of specifically preconfigured kits.
- The CX-1 pH gradient starter kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 10µm, 4 x 250mm column
- The CX-1 pH gradient high throughput kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 5µm, 4 x 50mm column
- The CX-1 pH gradient high resolution kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 5µm, 4 x 250mm column
