Recombinant Protein G - Sepharose® 4B

Catalog number: 101243

Novex™  Related applications: Antibody Purification | Protein Assays and Analysis | Protein Purification & Isolation

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Description

Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin).

Applications: Immunoglobulin affinity purification and Immunoprecipitation.

Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:

• all four subclasses of human IgG
• mouse IgG
• rabbit IgG
• guinea pig IgG
• rat IgG
• goat IgG
• sheep IgG
• cow IgG
• horse IgG

Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly to IgG from dog.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Suspension
Column Type: Affinity Column
Product Size: 25 mL
Immobilized Phase: Protein G
Shipping Condition: Wet Ice

Contents & storage

This product is supplied as a 50% suspension (25 ml Recombinant Protein G-Sepharose® 4B + 25 ml PBS)

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Manuals & protocols

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