Fetal Bovine Serum, qualified, USDA-approved regions

Citations & References (1)

Gibco™

Fetal Bovine Serum, qualified, USDA-approved regions

Name: Fetal Bovine Serum, qualified, USDA-approved regions
SKU: 10437028

Important Update: We've recently introduced Value Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Qualified USDA origin and Brazil (South American) origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.

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表示を変更

製品番号（カタログ番号）	数量	フォーマット
10437028	500 mL	Bottle
A3160601	50 mL	One Shot

2 オプション

Our improved Value Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.

製品番号（カタログ番号） 10437028

現在の製品を選択する

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Replacing with Fetal Bovine Serum, value
Sera application: for cell culture with robust cell lines, good value for basic research
Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
Hemoglobin level: ≤25 mg/dL
Origin: countries meeting USDA importation requirements

Gibco serum delivers

ISO 13485 certified, processed in FDA registered facilities
Triple filtered at 0.1 μm
Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

仕様

純度または品質グレード適格

出荷条件凍結

種Cattle/Bovine

Age胎児

原産国メキシコ

形状Liquid

フォーマットBottle

製品タイプFetal Bovine Serum

数量500 mL

Serum Treatment標準(滅菌ろ過済み)

無菌性Sterile

Sterilization MethodTriple-filtered, 0.1 μm

Unit SizeEach

組成および保存条件

保存条件：≤-10°C
輸送条件: 凍結

よくあるご質問（FAQ）

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the USDA Facility Approval Number for Fetal Bovine Serum, qualified, USDA-approved regions?

The Grand Island, NY facility has been assigned facility number NY-TEC-0001 by the USDA.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, qualified, USDA-approved regions FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human Fcgamma RIII and Antibody-dependent Cellular Toxicity.

Authors: Shields Robert L; Lai Jadine; Keck Rodney; O'Connell Lori Y; Hong Kyu; Meng Y Gloria; Weikert Stefanie H A; Presta Leonard G;

Journal:J Biol Chem

PubMed ID:11986321

Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1 that were deficient in fucose attached to the Asn(297)-linked carbohydrate but were otherwise similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from human serum. Lack of fucose on ... More