Search

  • Order Status
  • Quick Order
  • Promos
Fetal Bovine Serum, qualified, heat inactivated, Brazil - FAQs

Fetal Bovine Serum, qualified, heat inactivated, Brazil - FAQs

View additional product information for Fetal Bovine Serum, qualified, heat inactivated, Brazil - FAQs (10500056)

6 product FAQs found

When should I use heat-inactivated serum?

Heat-inactivation of serum inactivates the “complements” in the serum. In general, it is not necessary to use heat-inactivated serum to culture most cell types. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I would like to order heat-inactivated FBS. What conditions do you use for heat-inactivation of FBS during the manufacturing process?

Serum is heat inactivated by heating at 56 degrees C in a water bath for 30 min, swirling the bottle approximately every 10 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sometimes I notice flocculence in serum. What could it be?

Flocculence may appear in FBS for a variety of reasons. The most common reason is the denaturation of serum lipoproteins. You may observe fibrin, one of the clot-forming proteins present in serum, after the serum has been thawed. This should not affect product performance. To remove the flocculence, transfer the serum to sterile tubes and centrifuge the material briefly at 400 x g. Then filter the resulting supernant along with your media. Do not attempt to filter serum containing flocculence, it may clog filters.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does material precipitate in FBS upon storage in the refrigerator?

Gibco FBS is not pre-aged. When stored at 2 to 8 degrees C, the possibility exists for various proteins and lipoproteins in serum (e.g., cold agglutinins, fibrinogen, vitronectin, etc.) to aggregate, and form either perceptible material or observed turbidity. This should not affect serum performance. We recommend that you store FBS at -20 degrees C and avoid repeated freeze-thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.