Gibco™

DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

Green features
Catalog number: 10569010
Gibco™

DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

Green features
Catalog number: 10569010
Catalog Number
10569010
also known as 10569-010
Unit Size
500 mL
Price (USD)
37.71
Your Price:
Availability
***
Quantity
Catalog Number
10569044
also known as 10569-044
Unit Size
10 x 500 mL
Price (USD)
330.00
Your Price:
Availability
***
Quantity
Catalog Number
10569069
also known as 10569-069
Unit Size
5 L
Price (USD)
512.00
Your Price:
Availability
***
Quantity
Catalog Number
10569077
also known as 10569-077
Unit Size
10 L
Price (USD)
742.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10569010
also known as 10569-010
500 mL
37.71
Your Price:
***
10569044
also known as 10569-044
10 x 500 mL
330.00
Your Price:
***
10569069
also known as 10569-069
5 L
512.00
Your Price:
***
10569077
also known as 10569-077
10 L
742.00
Your Price:
***
Product Overview
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FAQ
Citations & References
Additional Information
Recommendations
Recommendations
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• HEPES
• Sodium Pyruvate
• GlutaMAX™
• Phenol Red

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (31966-021). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Concentration
1X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Sterility
Sterile-filtered
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™, GlutaMAX™
Form
Liquid
Glutamine
GlutaMAX-I
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Green Features
Sustainable packaging
Quantity
500mL
Cell Type
primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
With Additives
High Glucose, Sodium Pyruvate, GlutaMAX, Phenol Red
Without Additives
HEPES
Glucose
High Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
For Use With (Application)
Mammalian cell culture
Cell Line
HeLa, 293, Cos-7, and PC-12
Classification
Animal Origin-Free
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

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