For superior RT-PCR performance, upgrade to the new SuperScript IV One-Step RT-PCR System.
This product (Cat# 10928034) is being discontinued on April 5, 2019. For superior performance we recommend Cat# 12595025.
Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Catalog number:  10928034

 Related applications: Reverse Transcription

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Description

The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/ Platinum Taq Mix and 2X Reaction Mix. The RT/ Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription is automatically followed by PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript™ II
Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Detection Method: Gel Electrophoresis
Final Product Size(s): 3 kb or less
Primer-Probe Compatibility: Unlabeled Oligos
Product Line: Platinum®, SuperScript™
Product Size: 25 reactions
Shipping Condition: Wet Ice

Contents & storage

SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols