For superior PCR performance, upgrade to Invitrogen's new Platinum II Taq Hot-start DNA Polymerase.
Invitrogen™

Platinum™ Taq DNA Polymerase

Catalog number:  10966026

 Related applications:

Chromatin Biology

| Methylation Analysis

| PCR

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Description

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The "hot start" property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

Using Platinum Taq DNA Polymerase
The hot-start property of Platinum Taq DNA Polymerase allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.

Note: For superior PCR performance, we recommend next-generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Exonuclease Activity: 5' - 3'
Product Line: Platinum®
Product Size: 300 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Each box contains:
• 60 µL Platinum® Taq DNA Polymerase
• 2 × 1.25 mL 10X PCR Buffer (no MgCl2)
• 1 mL 50 mM MgCl2
• 1.25 mL KB Extender
Store at -30°C to -10°C in a non-frost-free freezer.

Documents

Manuals & protocols