Gibco™
Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation
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Description
Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:• Activation of T cells without the need for feeder cells
• Activated cells that retain in vivo-like function
• Maximum reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1,000-fold T cell expansion
About Dynabeads® Human T-Activator CD3/CD28
Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.
Starting samples
Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.
Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.
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Specifications
| Cell Type: | T Cells (Whole Population) |
|---|---|
| Clonality: | Monoclonal |
| Final Product: | Cells |
| Product Line: | DYNAL®, Dynabeads® |
| Product Size: | 2 mL |
| Reactivity: | Human |
| Sample Type (Specific): | Cell Cultures, PBMC |
| Target Species: | Human |
| Shipping Condition: | Room Temperature |
Contents & storage
Dynabeads® Human T-Activator CD3/CD28 are supplied as a suspension containing 4 × 107 Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, with 0.1% human serum albumin (HSA).
Storage Conditions: 2°C to 8°C. Do not freeze.
Storage Conditions: 2°C to 8°C. Do not freeze.
