Search
Invitrogen™
Dynabeads™ CD15
Dynabeads CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells directly from whole blood, buffy coat, or PBMC samples. Dynabeads CD15 can also be used to positively isolate CD15+ cells for molecular applications.
Have Questions?
Cambiar vista
| Número de catálogo | Cantidad |
|---|---|
| 11138D | 2 mL |
| 11137D | 5 mL |
Número de catálogo 11138D
Precio (MXN)
-
Cantidad:
2 mL
Dynabeads CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or peripheral blood mononuclear cell (PBMC) samples. Dynabeads CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads CD15:
- Efficient depletion of granulocytes (neutrophils and eosinophils)
- Isolation of granulocytes directly from whole blood for molecular applications
- No columns required
About Dynabeads CD15
Dynabeads CD15 are uniform, superparamagnetic polystyrene beads (4.5 μm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.
Magnetic bead-based separation offers easy handling
Dynabeads CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads to the target cells. By placing the sample on a magnet, the bead-bound target cells are separated from the rest of the sample in just 1 to 2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2 to 3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.
Automation-ready for use with KingFisher purification systems
The product is ideal for high-throughput workflows using KingFisher systems. A user guide and scripts for KingFisher Flex and Apex workflows are available for download on this page.
Learn more about Dynabeads products
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaGranulocytes
Compatibilidad de alto rendimientoYes
N.° de celdasProcesses ∼8x 10^8 cells total
Viabilidad de la salida>95%
Línea de productosDynabeads
Grado de pureza o calidadResearch Grade
Cantidad2 mL
Tipo de muestraPBMC, Blood
Duración de almacenamiento36 Months (from date of manufacture)
Condiciones de envíoRoom Temperature
N.º de celda del material de partida1 x 10^7 PBMCs per isolation
Especies dianaHuman
ColorBrown
Diámetro (métrico)4.5 μm
Para utilizar con (aplicación)Cell Depletion, Positive Isolation for Molecular Applications
MaterialMagnetic Polystyrene
Unit SizeEach
Contenido y almacenamiento
This Product Contains: 2 mL Dynabeads™ CD15 coated with anti-CD15 monoclonal antibody.
Storage Conditions: 2°C to 8°C. Do not freeze.
Storage Conditions: 2°C to 8°C. Do not freeze.