CD Hybridoma Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  11279023

 Related applications:

Antibody & Cell Culture Production

| Mammalian Cell Culture

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CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:

• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin–free, protein-free, and chemically defined formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin–free, protein-free, and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Product use
Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco® CD Hybridoma Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Hybridomas, Myeloma Cells
Classification: Animal Origin-Free, Chemically Defined, Protein-Free
Form: Liquid
Glutamine: No Glutamine
Phenol Red Indicator: No Phenol Red
Product Line: Gibco®
Product Size: 1,000 mL
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture


Manuals & protocols