Invitrogen™

ElectroMAX™ DH5α-E Competent Cells

Catalog number: 11319019
Invitrogen™

ElectroMAX™ DH5α-E Competent Cells

Catalog number: 11319019
Catalog Number
11319019
Unit Size
5 x 100 µL
Price (USD)
334.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
113190195 x 100 µL
334.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as generation of cDNA libraries or in transformations with limited input DNA. ElectroMAX DH5α-E cells offer:

• >1 x 1010 transformants/µg efficiency with electroporation
• Greatly increased plasmid yield and quality due to endA1 mutation
• High-efficiency transformation with plasmids 30 kb in size
• Blue/white screening of recombinant clones due to lacZΔM15
• Ensured insert stability due to recA1 mutation
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
DH5α
Blue/White Screening
Yes
Cloning Methylated DNA
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Competent Cell Type
Electrocompetent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
May be used for plasmids > 20 kb
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Transformation Efficiency
> 1x10^10
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Format
Tube(s)
Product Line
DH5a, ElectroMAX™
Product Type
Competent Cells
Species
E. coli
Improves Plasmid Quality
Yes

Contents & Storage

Contains:
• ElectroMAX DH5α-E Competent Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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