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SuperScript® Human Skeletal Muscle cDNA Library - FAQs

View additional product information for SuperScript® Human Skeletal Muscle cDNA Library - FAQs (11327012)

4 product FAQs found

Is the sequence of the CMV promoter in pcDNA3.1 vector complete, or is it only the core CMV promoter?

In addition to the CAAT and TATA boxes, the CMV promoter in pcDNA3.1 vector contains sequence homologous to base pairs 137 to 724 of the sequence submitted by Boshart, et al (GenBank Accession # K03104). The complete enhancer region is contained between 214 and 620 of this sequence. Therefore, by this definition, the CMV promoter could be said to be "complete".

Please note that this promoter does not contain an intron. Some people believe that the complete promoter must contain the intron, but that has not been demonstrated to be necessary for expression.

Do Invitrogen cDNA libraries contain attB or attL sites?

It depends upon the library. Only libraries in pCMVSport6 or pCMVSport6.1 have attB sequences.

Can I transfect an amplified SuperScript cDNA library directly into eukaryotic cells?

Yes, but the pCMVSport vector does not have a eukaryotic origin of replication or selectable marker, so you can only screen for transient expression.

Can I use antibodies to screen SuperScript cDNA libraries?

You can only do this if you transfect the DNA into eukaryotic cells. There is no bacterial promoter in the pCMVSport vector.