Gibco™

DMEM/F-12, HEPES

Green features
Catalog number: 11330099
Gibco™

DMEM/F-12, HEPES

Green features
Catalog number: 11330099
Catalog Number
11330099
also known as 11330-099
Unit Size
5 L
Price (USD)
440.00
Your Price:
Availability
***
Quantity
Catalog Number
11330057
also known as 11330-057
Unit Size
10 x 500 mL
Price (USD)
370.00
Your Price:
Availability
***
Quantity
Catalog Number
11330032
also known as 11330-032
Unit Size
500 mL
Price (USD)
43.19
Your Price:
Availability
***
Quantity
Catalog Number
11330107
also known as 11330-107
Unit Size
10 L
Price (USD)
698.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11330099
also known as 11330-099
5 L
440.00
Your Price:
***
11330057
also known as 11330-057
10 x 500 mL
370.00
Your Price:
***
11330032
also known as 11330-032
500 mL
43.19
Your Price:
***
11330107
also known as 11330-107
10 L
698.00
Your Price:
***
Product Overview
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FAQ
Citations & References
Additional Information
Recommendations
Recommendations
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:
With
• L-glutamine
• HEPES
• Phenol Red

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31330-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

Concentration
1X
Product Type
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterility
Sterile-filtered
Serum Supplementation
Standard Serum Supplementation
Shipping Condition
Room Temperature
Volume (Metric)
5 L
Product Line
Gibco™, SynerGy™
Form
Liquid
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Shelf Life
12 Months From Date of Manufacture
Quantity
5L
With Additives
L-Glutamine, HEPES, Phenol Red
Glucose
High Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
For Use With (Application)
Antibody and cell culture production, Mammalian cell culture
Cell Line
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Classification
Animal Origin-Free
Solution Type
DMEM (Dulbecco's Modified Eagle Medium)/F-12
HEPES Buffer
HEPES

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations

Figures