Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched B cells • Easy to use and to scale up • No columns required
An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.
Starting Sample: PBMC
For Research Use Only. Not for use in diagnostic procedures.
Final Product Type
No. of Cells
Processes ∼1x10^9 cells⁄kit
No. of Tests
DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade
Starting Material Cell No.
Contents & Storage
The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads™ and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A). Store at 2-8°C