Dynabeads® Mouse CD43 (Untouched™ B Cells)

Catalog number: 11422D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

High purity, recovery and viability of mouse untouched B cells

• Easy to use and to scale up
• No columns required

Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
spleen or lymph nodes
For Research Use Only. Not for use in diagnostic procedures.


Form: Bead
Capacity: Processes ~2x10^9 cells total, Processes ~2x10^9 cells⁄kit
Cell Type: B Cells
Reactivity: Mouse
Product Size: 1 kit
Final Product: Cells
Target Species: Mouse
Isolation Method: Negative Isolation
Number of Reactions: 40 Tests
Sample Type (Specific): Spleen, Lymph Node, Tissue Digests
Starting Material (Cell #): 5x10^7 cells⁄test
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 5 ml MyOne™ Dynabeads®- coated with streptavidin and biotinylated rat monoclonal CD43 antibody
against mouse T cells, monocytes⁄macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.
Store at 2-8°C


Manuals & protocols

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