Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.
High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up • No columns required
Methodology: Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.
Starting Sample: spleen or lymph nodes
For Research Use Only. Not for use in diagnostic procedures.
The Kit Contains: 5 ml MyOne™ Dynabeads™- coated with streptavidin and biotinylated rat monoclonal CD43 antibody against mouse T cells, monocytes⁄macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. Store at 2-8°C