Dynabeads® Mouse DC (Dendritic Cell) Enrichment

Catalog number: 11429D

Invitrogen™  Related applications: Cell Isolation & Expansion

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Use this kit to enrich viable mouse dendritic cells (DC's) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DC's are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.

Obtain high recovery (80-100%) of viable Lin-CD11c+ DC's of all sup-populations, including DC's positive for CD4, CD8α, B220 and CD19.

• Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.
• Pool cells/tissue for easy sample scale -up

Methodology:
An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC's can be directly sorted into the sup-population of choice or used in any other application.

Starting samples:
Spleen or lymph node cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~1x10^9 cells total
Cell Type: Dendritic Cells
Reactivity: Mouse
Product Size: 5 mL
Target Species: Mouse
Isolation Method: Negative Isolation
Output Viability: >95%
Sample Type (Specific): Spleen, Lymph Node
Starting Material (Cell #): 1x10^7 cells per isolation
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 5 ml Depletion MyOne™ Streptavidin Dynabeads® and 1 ml Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards mouse CD2, CD3ε, CD49b, mIgM and Ter-119.
Store at 2-8°C

Documents

Manuals & protocols

Recommended products