Gibco™

Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation

Catalog number: 11453D
Gibco™

Dynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation

Catalog number: 11453D
Catalog Number
11453D
Unit Size
5 x 2 mL
Price (USD)
1,974.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
0.4 mL
Price (USD)
175.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
2 mL
Price (USD)
772.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11453D5 x 2 mL
1,974.00
Your Price:
***
11456D0.4 mL
175.00
Your Price:
***
11452D2 mL
772.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Dynabeads™ Mouse T-Activator CD3/CD28 is for the activation and expansion of mouse T-cells for research purposes.

With Dynabeads™ Mouse T-Activator CD3/CD28 you get:

• Activation of T-cells without the need of feeder cells
• Activated cells that retain in vivo-like function
• Maximal reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1000-fold T-cell expansion

How Dynabeads™ Mouse T-Activator CD3/CD28 works:
Dynabeads™ Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.

For expansion of antigen specific T-cells from T-cell clones or T-cell lines, we recommend using Dynabeads™ Mouse T-Activator CD3/CD28/CD137.

Starting samples
Start with single cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T-cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. Get an overview of the different Dynabeads™ available for cell isolation.

Downstream applications
The activated T-cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T-cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

Specifications

Cell Type
T Cells (Whole Population)
Clonality
Monoclonal
Product Type
Mouse T-Activator CD3/CD28
Final Product Type
Cells
Product Line
DYNAL™, Dynabeads™
Purity or Quality Grade
Research Grade
Quantity
5 x 2 mL
Reactivity
Mouse
Sample Type
Cell Cultures, Lymph Node, Spleen
Shipping Condition
Room Temperature
Target Species
Mouse
Diameter (Metric)
4.5 μm

Contents & Storage

5 x 2 ml Dynabeads™ Mouse T-Activator CD3/CD28 is supplied as a suspension containing 4 × 107 Dynabeads™/ml in phosphate buffered saline (PBS), pH 7.4, w/0.1% bovine serum albumin (BSA). Store at: 2°C to 8°C.

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